Determination of Protein Primary Structure

The primary structure of a protein is the foundation of its biological function, and determining its primary structure aids in studying the protein's function. Biotech company Biotyper offers mass spectrometry-based protein primary structure analysis services.

Protein Structure

Protein structure is the three-dimensional arrangement of atoms within a molecule of an amino acid chain. To perform its biological function, a protein folds into one or more specific spatial conformations. There are four levels of protein structure: primary structure, which refers to the sequence of amino acids in a polypeptide chain, including the positions of disulfide bonds; secondary structure, which refers to highly regular local sub-structures such as α-helices and β-sheets on the actual polypeptide backbone; tertiary structure, which is the three-dimensional structure formed by the spatial arrangement of multiple secondary structures; and quaternary structure, which is the three-dimensional structure formed by the assembly of two or more polypeptide chains (subunits) that function as a single unit. The primary structure of a protein is the foundation of its biological function, and determining its primary structure is fundamental to understanding protein structure and function.

Determination of Protein Primary Structure

Determination of protein primary structure mainly includes measuring the number of polypeptide chains, the type, number, and sequence of amino acids in each polypeptide chain, and the position and number of disulfide bonds within or between polypeptide chains. When measuring the primary structure of a protein, the purity of the protein must be over 97%, and the molecular weight of the protein needs to be determined first. The steps for protein primary structure analysis mainly include the separation and degradation of polypeptide chains, the separation and sequence analysis of peptides, and the localization of disulfide bonds. Mass spectrometry techniques can be used for determining the primary structure of proteins, includingMolecular Weight Determination,Amino Acid Composition Analysis,Peptide Sequence AnalysisandDisulfide Bond Localization, etc.

Determination of Protein Primary Structure

Related Services:

Protein Molecular Weight Determination
Amino Acid Composition Analysis
Mass Spectrometry-Based Sequence Analysis
Identification and Quantitative Analysis of Protein Disulfide Bonds