High-Throughput Gene Knockout Proteomics Analysis
Proteomics is a scientific field that studies the composition, structure, function, and interactions of all proteins in a biological organism. Its goal is to establish a comprehensive list of proteins and understand their roles in life processes. However, proteins with fully studied biological functions are still relatively few, and some protein-coding genes remain unannotated. The vast diversity of protein types produced by nonsynonymous single nucleotide polymorphisms, alternative splicing, and post-translational modification processes makes this research more challenging. The emergence of high-throughput gene knockout technology provides a powerful tool for large-scale detection of proteins and protein phenotypes. High-throughput gene knockout technology can be used to silence or knock out specific genes, and observe changes in the proteome. Combined with high-resolution mass spectrometry, it can be used to analyze protein composition, modifications, and relative abundance.
Dolgalev G, et al. Genes. 2021.
Application of High-Throughput Gene Knockout Technology in Proteomics
The combination of high-throughput gene knockout technology and proteomics offers a new perspective for biological research. By integrating these two technologies, researchers can study the impact of specific gene knockouts on the proteome, identify proteins associated with the knocked-out gene, and thereby better understand the interactions between genes and proteins, revealing protein regulatory networks. For example, researchers can determine how the knockout of a specific gene affects protein expression in cancer cells, potentially identifying new cancer treatment strategies. Additionally, this combination can be used to study the mechanisms of various diseases, such as neurodegenerative diseases, immune diseases, and metabolic disorders.
Based on a high-resolution proteomics analysis platform, BTP (Biotechnology) offers an integrated solution from gene knockout to proteomics analysis by combining high-throughput gene knockout technology. Our optimized CRISPR/Cas9 system enables single/multiple gene knockout, frameshift mutations, and large sequence deletions in human/mouse cell lines, primary cells, immune cells, and iPS cells. After obtaining KO cells, we culture them and extract the proteins expressed by the KO cells. Using a high-resolution mass spectrometry platform, we conduct proteome analysis, interpreting the role of specific knocked-out genes at the protein level and uncovering the mechanisms by which genes regulate proteins. BTP has established seven major testing platforms and has laboratories with dual CNAS/ISO9001 quality system certifications, aiming to provide you with the highest quality scientific research services. We look forward to collaborating with you. Please contact us for more service details!
Service Advantages
1) Knockout Efficiency Guarantee: Optimized CRISPR/Cas9 system with over 80% of target sites achieving a knockout efficiency of 70%. After gene knockout, we use Sanger sequencing and in-depth proteomics techniques for dual validation of knockout efficiency, ensuring data authenticity with double assurance.
2) Comprehensive Proteomics Analysis Platform: Our proteomics analysis platform is equipped with comprehensive sample pre-processing instruments, a high-specificity protein modification enrichment platform, ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry, and a protein N-terminal sequencing analyzer. It enables efficient extraction of proteins at the omics level, high-efficiency enrichment of specific modified peptides, and identification, relative quantification (3D & 4D), and absolute quantification analysis of proteins, providing a comprehensive analysis of the proteome expressed by KO cells.
3) Short Project Cycle:Before conducting gene knockout experiments, we analyze gene necessity and expression levels in different cells to ensure the feasibility of the experiments, reducing the risk of experimental failure. Additionally, there is no need for pre-experiment gRNA validation for the customer's target, significantly shortening the project cycle.
4) One-Stop Service:BTP has extensive experience in omics services and professional technical personnel. We can customize the most optimal project plan according to your needs. You only need to tell us your experimental objectives and send the samples. BTP will handle all subsequent projects, including sample processing, experimental analysis, data analysis, and project reporting.
Application Areas
1.Disease Mechanism Research
By knocking out specific genes, researchers can observe changes in protein expression and regulation under disease conditions. For example, in cancer research, knocking out genes related to cancer development can lead to a better understanding of the biological mechanisms of tumors.
2.Drug Target Discovery
By analyzing the impact of gene knockout on the proteome, new drug targets can be identified. For example, in the search for new treatments for Alzheimer's disease, researchers can knock out genes associated with cognitive decline to find potential therapeutic targets.
3.Functional Genomics Research
By combining gene knockout technology and proteomics, researchers study the functions of genes and proteins in cells, as well as how they interact to regulate biological processes.
4.BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
In the field of systems biology, combining gene knockout technology and proteomics helps establish protein-protein interaction networks, aiding in the understanding of complex biological systems. For example, researchers can reveal key regulatory networks in disease processes by analyzing changes in the proteome under specific pathological conditions.
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High-Throughput Gene Knockout Services
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