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O-glycosylation site analysis

O-glycosylation isIn addition to N-glycosylation, anotherimportant type of proteinglycosylationmodification,Unlike N-glycosylation, O-glycanchainsare usuallyattached toSerine (Ser) or Threonine (Thr)on residues. O-glycosylation site analysis typically involves multiplesteps, including protein extraction, protein digestion, enrichment of O-glycosylated peptides, and liquid chromatography-mass spectrometry analysis. By analyzing O-glycosylation sites, detailed information about the protein's O-glycosylation status can be obtained, as well ashow this post-translational modification affects the function and activity of proteins, therefore, inresearch and development of new drugs, as well as the production and optimization of protein-based therapeutics, etc.the field has also gainedApplication.

Bai Tai Peike Biotechnology BTPCertified by CNAS/ISO9001 dual quality management systems. The companyBased on the principle of glycosylation site analysis, utilizing high-efficiencyliquid chromatographyCombinationHigh-Resolution Mass Spectrometry (LC-MS/MS)EstablishedStable and EfficientGlycosylation Site Analysis Platform, provides O-glycosylation site analysis services for biological products such as proteins/peptides, antibodies, protein vaccines, and recombinant collagen, including Oglycan analysis、Oglycosylation site identificationas well asa complete analysis process for glycopeptide analysis.Welcome to inquire for free to learn more details!

Experimental Instruments

NanoLC: Vanquish NEO
MS: Orbitrap Fusion Lumos With ETD

Case Illustration

We use a direct analysis method of O-glycopeptides for O-glycosylation site analysis. First, according to the theoretical amino acid sequence information of the protein, an appropriate protease is selected to cleave the protein into peptides. Then, raw mass spectrometry data is collected by liquid chromatography-high resolution mass spectrometry. This data is imported into the O-glycan database, and O-glycan modification is added as a variable modification in the data analysis. Finally, the sites within the protein where O-glycosylation modifications have occurred are identified.

Figure 2 Secondary Graph of O-Glycosylation Sites

Note: The O-glycosylation modification occurs at the fifth amino acid of the peptide segment, with a molecular weight change of +365.132. The x-axis represents the mass-to-charge ratio, and the y-axis represents signal intensity.

Bilingual Project Report

In the technical report, BiotechPack will provide you with a detailed bilingual (Chinese and English) technical report, which includes:

1. Experimental Procedures (Chinese and English)
2. Relevant Mass Spectrometry Parameters (Chinese and English)
3. Detailed Information on O-Glycosylation Site Analysis
4. Mass Spectrometry Images
5. Raw Data

O-Glycosylation Site AnalysisOne-stop Service

You Only Need to Order - Send Samples
BiotechPack's One-stop Service Includes: Sample Processing - Instrument Analysis - Data Analysis - Project Report