Glycosylation Site Analysis

The analysis of glycosylation sites in glycosylated proteins is of great significance for studying protein structure and function.

Biotech Pack offers glycan relative content analysis services based on LC-MS/MS. Multiple enzymes are first selected for protein digestion, followed by labeling with H2₂¹⁸O. PNGase F or multiple O-glycosidases are used to cleave glycans from the target protein, at which point glycosylated peptides will carry an¹⁸O label. Database searches can then identify glycosylation sites.

Glycosylation Site Analysis

Technical Features

• Relatively short analysis time
• High detection sensitivity and good reproducibility

Application Areas

• Identification of N-glycosylation sites in glycosylated proteins
• Identification of O-glycosylation sites in glycosylated proteins

About Samples

Glycosylation Site Analysis Sample Requirements

1. If you are providing tissue samples, please ship them to us on dry ice.
2. If you are providing protein samples, use regular tissue or cell lysis buffer for protein extraction.
3. Sample Shipping: Use sufficient dry ice for shipping and select a faster shipping method to minimize the risk of sample degradation during transit.

Bilingual Project Report

In the technical report, Biotech Pack will provide you with a detailed bilingual report, which includes:

1. Experimental procedures (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Identification of N-type and O-type glycosylation sites
4. Mass spectrometry images
5. Raw data

One-stop Glycosylation Site Analysis Service

You only need to place an order and send samples.

Biotech Pack completes the one-stop service: sample processing - on-machine analysis - data analysis - project report

Biotech Pack offers the following glycosylation site analysis services

Related Services

Biopharmaceutical Glycosylation Site Detection
Glycan Analysis
Biopharmaceutical Glycan Profiling
Glycosylation Site and Glycan Type Analysis at the Site
Quantitative Glycoproteomics Research
Glycoprotein Analysis