HPLC Protein Analysis
High-Performance Liquid Chromatography（HPLC，High Performance Liquid Chromatography）is a technique that utilizes the chemical and physical interactions between the stationary phase (particles within the chromatography column) and the mobile phase (solution)，to achieve high resolution and high sensitivityin achievingthequalitative and quantitative analysis of proteinstechnology，infields such as biological sciences, drug development, and quality controlhas a wide range of application prospects. WithHPLCFrom the early experimental stages to the development of modern diverse technologies in the field of protein analysisstages,HPLCnot only can it provide support forprotein research, but it can also help reveal the complexity of biological systems, identify new drug targets, and optimize the production processes of biological products.
HPLCThe mainmethods of protein analysis include reversed-phase high-performance liquid chromatography（RP-HPLC）, ion exchange chromatography（IEX）, size exclusion chromatography（SEC）and affinity chromatography（AC）。RP-HPLCwhich utilize hydrophobic interactions to separate proteins, suitable for protein purity identification and peptide analysis.IEXSeparation is achieved through differences in protein charge, allowing the determination of the isoelectric point and charge distribution of proteins.SECSeparation is based on protein molecular size, assessing molecular weight, polymers, and subunit composition.ACProtein separation is achieved through specific biomolecular recognition, commonly used in studying protein-ligand interactions.
Bio-TechneHPLCGeneral Procedure for Protein Analysis
1.Sample Processing
We perform necessary pretreatment on received samples, such as impurity removal, concentration, and desalting, to improve the accuracy and reproducibility of the analysis.
2. HPLC Method Optimization
Based on protein characteristics and customer requirements, select appropriate chromatography columns, mobile phase, and detector parameters, and set conditions such as flow rate, mobile phase composition, and temperature.。
3. HPLC Analysis
Analyze the samples using the optimized HPLC method, record and interpret chromatograms to obtain information on protein separation, purity, and concentration.
4.Data Processing and Analysis
Use dedicated software for peak identification, quantification, and characterization of chromatographic data。
OurAdvantages
1.High Resolution：HPLCThe technology offers high resolution, enabling accurate separation among multiple proteins,Effective SeparationProteins with similar structures or functions。
2.High SensitivityAccuracy:Capable ofdetecting protein concentrations as low as nanograms。
3.ReproducibilityGood:High stability and reliability of experimental results.
4.Strong contamination resistance：Tolerates impurities in samples to some extent, reducingthe purity requirementsof the samples.
5.Wide Applicability：By selecting appropriate chromatographic columns and mobile phases,it can accommodate various types of biological samples such as serum, tissue extracts, and cell lysates, providing comprehensive services for clients.
6Customizable: Our technicians can optimize analytical methods based on customer needs to enhance separation efficiency and meet specific research goals.
HPLCProtein Analysis Technology Applications
1.Biopharmaceuticals
Applied in drug development, production, and quality control to evaluate key parameters such as the purity, activity, and stability of drug proteins.。
2.Proteomics
Used for the separation, identification, and quantification of proteomes, achieving high-throughput analysis in combination with mass spectrometry technology.。
3.Biomarker Research
Discovery and validation of potential biomarkers related to diseases or physiological processes。
4.Food safety and quality control
Detection of protein components, additives, contaminants, etc., in food to ensure food safety and quality。
5.Disease diagnosis
Analysis of protein changes in biological samples to assist in disease diagnosis and assessment。
About samples
|
Sample type |
Cell extracts |
Tissue extracts |
Serum/Plasma |
Urine |
Extracellular Vesicles |
Amino Acid Solution |
|
Sample Quantity |
100-1000ug |
100-1000ug |
10-100ul |
100-1000ul |
10-100ug |
10-1000ug |
*Please avoid using reagents that may interfere with HPLC analysis, such as high concentrations of salts, acids, bases, dehydrating agents, etc. If needed, we can provide sample processing for you.
InEnglish Project Report
In the technical report, BiotechPack will provide you with a detailed bilingual (Chinese and English) version of the technical report, which includes:
1.Experimental Procedures (Chinese and English)
2.Relevant Chromatographic Parameters (Chinese and English)
3.Protein HPLCAnalysisDetails
4. ColorSpectrum Images
5.Raw Data
HPLCProtein AnalysisOne-Stop Service
You only need to place an order and send samples
BiotechPack One-Stop Service includes: Sample Processing - Instrumental Analysis - Data Analysis - Project Report
Related Services
Characterization of Protein Purity and Homogeneity
Protein Purity Analysis (Size Exclusion/Reverse Phase Chromatography)
Peptide Mass Spectrometry Identification
How to order?
