HPLC Determination of Protein Purity

Protein purity refers to the relative proportion of the target protein in a protein sample. It is usually expressed as a percentage, with higher purity indicating a higher proportion of the target protein in the sample. Measuring protein purity is an important aspect in fields such as biochemistry, molecular biology, and biotechnology. By assessing protein purity, the quality of extracted protein samples can be evaluated, providing a reference for subsequent experimental operations. High-purity protein samples help ensure the reliability of experiments, especially when studying the function, structure, and interactions of proteins. In the biopharmaceutical industry, protein purity is a critical parameter. Measuring protein purity can optimize production processes and improve product quality.

The process of determining protein purity using HPLC at Biotai Parker Biotechnology

1.Select the appropriate HPLC column:

Choose the appropriate chromatographic column based on the characteristics of the protein (molecular weight, polarity, hydrophobicity, etc.).

2.Prepare the sample:

Process the purified protein sample according to experimental requirements, such as adjusting concentration, pH, ionic strength, etc., to avoid protein degradation, aggregation, etc.

3.Set HPLC conditions:

Set appropriate parameters for the mobile phase, flow rate, gradient program, detection wavelength, etc., based on the protein characteristics and the type of chromatographic column selected.

4.Inject the sample:

Inject the processed protein sample into the HPLC system for chromatographic analysis.

5.Analyze the results:

Observe the number, shape, and relative area of peaks in the chromatogram to assess protein purity.

6.Quantitative calculation:

Calculate the protein purity based on the area or height of chromatographic peaks and the detection wavelength of the protein.

Weofferadvantages

1. High precision and high sensitivity:

We employ advanced high-performance liquid chromatography (HPLC) technology to accurately detect protein samples within a very low concentration range, ensuring the accuracy and reliability of results.

2. Wide application range:

HPLC methods are suitable for various types of protein samples, including natural proteins, recombinant proteins, modified proteins, etc. Whether it is a single protein or a protein mixture, we can provide accurate purity analysis.

3. Short experimental cycle:

Our HPLC system features high resolution and rapid analysis capabilities, allowing us to complete protein purity detection in a short time frame to meet customer time requirements.

4. Professional technical team:

We have an experienced technical team that can select the appropriate HPLC column and method for protein purity determination based on customer needs, ensuring the accuracy and reliability of results.

5. Data processing and reporting:

We provide detailed data processing and analysis reports, including chromatograms, purity percentages, etc., for customers to quickly understand and evaluate experimental results.

6. Customized service:

We can offer customized HPLC protein purity determination services, including sample processing, column selection, method optimization, etc., to meet the needs of different application scenarios.

About the samples

*Please use buffers suitable for HPLC analysis. Avoid using buffer components that may have adverse effects on the chromatography column, such as high concentration salts, chelating agents, non-volatile buffers, lipids, detergents, etc.

Bilingual project report (English/Chinese)

In the technical report, Biotai Parker will provide you with a detailed bilingual technical report in both English and Chinese, including:

1. Experimental procedures (English/Chinese)
2. Relevantchromatographicparameters (English/Chinese)
3. HPLCdetailed information on protein purity determination
4. Chromatogramimages
5. Raw data

HPLCOne-stop service for determining protein purity

You only need to order and send your samples
Biotai Parker provides a one-stop service: sample processing, machine analysis, data analysis, project report

Related services

SDS-PAGE protein purity analysis

Protein purity analysis (size exclusion/reversed-phase chromatography)

Protein isoelectric point determination

Protein purity and homogeneity characterization

Protein structure identification

Molecular weight determination