Acetylated Quantitative Proteomics Research

Quantitative Acetylation Proteomics Study

Acetylation modification is a highly conserved reversible protein modification in the body, playing a crucial role in the activation of transcriptional regulators within the cell nucleus. Additionally, a large number of non-histone acetylation modifications are involved in the regulation of metabolic pathways and metabolic enzyme activities.

Due to the low abundance and wide dynamic range of acetylated proteins in biological samples, enrichment of acetylated peptides is required prior to mass spectrometry analysis to enhance their abundance. Traditional quantitative proteomic analysis techniques are then used to quantify the enriched acetylated peptides. BTP Company utilizes peptide pre-separation to reduce the impact of high-abundance histones on protein acetylation identification. This is combined with immunoprecipitation using highly efficient antibody enrichment of acetylated peptides, enabling large-scale acetylation identification and quantification. The selected kits are internationally recognized acetylated peptide-specific enrichment kits provided by CST Company. To identify and quantify more acetylated peptides, we use 2-3 different enzymes for protein digestion during the enzymatic digestion process, ensuring that peptides with acetylation sites are efficiently ionized and introduced into the mass spectrometer for analysis, avoiding the loss of acetylation information due to unsuitable peptide length or low ionization efficiency.

BTP Company utilizes Thermo Fisher's Q Exactive HF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, and Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC, offering an acetylation quantitative proteomics analysis service package. You only need to inform us of your experimental objectives and send your samples to us. We will handle all subsequent project matters, including protein extraction, protein digestion, acetylated peptide enrichment, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

Acetylation Quantitative Proteomics Analysis Workflow

Acetylation Modification Research Route

About Samples

Acetylation Quantitative Proteomics Analysis Sample Requirements

1. If you provide tissue samples, please send them to us on dry ice.
2. If you provide protein samples, use ordinary tissue or cell lysis buffers for protein extraction.
3. Sample transportation: Please use sufficient dry ice for transportation and choose faster shipping methods to minimize the risk of sample degradation during transport.
4. Before the formal experiment, we will test the samples you provide. Formal experiments will only begin if the samples pass the test.

Research Cases

This project involves the identification of protein ubiquitination modification sites in Co-IP samples from a teacher at the Institute of Biophysics, Chinese Academy of Sciences, conducted at BTP. Part of the results is shown in the figure below:

Acetylation Modification Research Case

Project Report in English/Chinese

In the technical report, BTP will provide you with a detailed bilingual technical report in both English and Chinese. The report includes:

1. Experimental steps (in English and Chinese)
2. Related mass spectrometry parameters (in English and Chinese)
3. Detailed information on identified acetylation sites
4. Mass spectrometry images
5. Raw data

One-stop Acetylation Quantitative Proteomics Service

You only need to place an order and send the samples

BTP One-stop Service Completion: Sample Processing - On-machine Analysis - Data Analysis - Project Report

Related Services

Post-translational Modification Proteomics Analysis
Histone Post-translational Modification Analysis
Quantitative Proteomics Analysis
Targeted Proteomics
4D Proteomics
DIA Quantitative Proteomics
Label-free Quantitative Proteomics Analysis
Label-based Protein Quantification Techniques - iTRAQ, TMT, SILAC
MRM/PRM Quantitative Proteomics Analysis