Small ubiquitin-like modifier (SUMO) protein modification is a type of post-translational modification that involves covalently attaching a small ubiquitin-related modifier (SUMO) to a protein. Unlike ubiquitination, SUMOylation does not target proteins for proteolysis but regulates protein functions in various cellular processes, such as nucleocytoplasmic transport, transcription regulation, apoptosis, protein stability, stress response, and cell cycle progression. Despite the low amino acid sequence similarity between SUMO and ubiquitin, they have nearly identical structural folds. SUMO is about 100 amino acids long, and the sequence length and mass of SUMO differ among SUMO family members and across organisms.
SUMOylation is involved in regulating the subcellular localization of many substrate proteins. Depending on the target protein, SUMOylation can occur in the cytoplasm or the nucleus. For example, the addition of SUMO can regulate the transport of ribonucleoprotein RanGAP1 in the nuclear pore complex (NPC). SUMOylation also regulates transcription processes. The activity of many transcription factors is related to their interaction with PML and nuclear bodies (NB), and the assembly of PML and NB requires SUMOylation of PML proteins. Moreover, SUMOylation plays an important role in chromosome condensation and kinetochore assembly. Dysfunctions of SUMO-1 can disrupt the correct distribution of chromatin in replicating cells.
Protein SUMOylation Identification
BrightGene Biotech uses the Thermo Fisher Orbitrap Fusion Lumos mass spectrometry platform, combined with nanoLC-MS/MS nano liquid chromatography, to provide a protein SUMOylation analysis service package. You only need to tell us your experimental objectives and send us your samples, and we will take care of all subsequent project matters.
General Process for SUMOylation Identification Service
1. Digest proteins in gel or solution;
2. Enrich SUMOylated proteins using antibodies targeting specific SUMOylation motifs;
3. Separate using HPLC, followed by ESI-TOF MS/MS analysis;
4. Mass spectrometry data analysis.
BrightGene provides the following bioinformatics services
