Tyrosine Phosphorylation Quantitative Proteomics Analysis

Protein phosphorylation is a common post-translational modification in eukaryotes. In eukaryotic cells, protein phosphorylation is divided into two types: serine/threonine phosphorylation and tyrosine phosphorylation (pY). Although tyrosine phosphorylation accounts for a smaller proportion, it plays a highly significant role in the fate of cells and organisms, being closely related to diseases such as cancer. Studies have shown that tyrosine kinase genes account for 75% of oncogenes, and 63% of protein kinase-targeted cancer drugs are also tyrosine kinase inhibitors.

 

Quantitative proteomics analysis of tyrosine phosphorylation is an efficient method for in-depth study of tyrosine phosphorylation events in cell signal transduction. Its principle mainly involves the use of techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately identify and quantify tyrosine phosphorylated proteins, revealing tyrosine kinases related to major diseases like cancer, thereby aiding in the discovery of tyrosine kinase inhibitors. In quantitative proteomics analysis of tyrosine phosphorylation, the enrichment of tyrosine phosphorylated peptides is the most crucial step. BTP (BioTopia) can choose antibody-affinity enrichment or SH2 superbinder enrichment according to experimental needs to efficiently enrich samples, and can achieve stepwise separation of mono-phosphorylated and multi-phosphorylated peptides, avoiding the impact of ionization efficiency issues during mass spectrometry analysis on the identification and quantification of mono-phosphorylated peptides.

BTP - Tyrosine Phosphorylation Quantitative Proteomic Analysis Workflow

 

BioTopia (BTP) is certified by the dual quality systems of CNAS/ISO9001. The company has established a tyrosine phosphorylation quantitative proteomics analysis platform based on Thermo's Orbitrap Fusion Lumos mass spectrometer combined with Nano-LC nano-liquid chromatography technology. Whether you are engaged in basic research or biopharmaceutical development, our tyrosine phosphorylation quantitative proteomics analysis services will provide strong support and accurate data for your research.

Feel free to contact us for a complimentary consultation and learn more details!

 

Bilingual Project Report (Chinese/English)

In the technical report, BioTopia will provide you with a detailed bilingual (Chinese and English) technical report, which includes:

1. Experimental procedures (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Detailed information on tyrosine phosphorylation analysis
4. Mass spectrometry images
5. Raw data

 

Tyrosine Phosphorylation Quantitative Proteomics AnalysisOne-stop Service

You only need to place an order and send samples
BioTopia's one-stop service completes: sample processing, on-machine analysis, data analysis, and project reporting

 

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