Phosphorylation Quantitative Proteomics Research

BTP-Phosphorylation Quantitative Proteomics Research

Phosphorylation modifications are simple, flexible, and reversible, with the accessibility of ATP as a phosphate group donor, making it a common regulatory mechanism in eukaryotic cells. The reversible process of protein phosphorylation and dephosphorylation regulates nearly all life activities, including cell proliferation, development, differentiation, cytoskeleton regulation, apoptosis, neural activity, muscle contraction, metabolism, and tumorigenesis.

Due to the low abundance and wide dynamic range of phosphorylated proteins in biological samples, quantitative proteomics analysis of enriched phosphorylated peptide samples requires enrichment to increase the abundance of modified proteins. Based on experimental needs, BiotechPack selects solid-phase metal affinity chromatography (IMAC), phospho-specific antibody enrichment, or TiO2 enrichment to achieve efficient sample enrichment. During the peptide enrichment process, mono-phosphorylated and multi-phosphorylated peptides are stepwise separated for mass spectrometry analysis, avoiding the impact of ionization efficiency issues of multi-phosphorylated peptides on the identification and quantification of mono-phosphorylated peptides during mass spectrometry analysis.

BiotechPack utilizes Thermo Fisher's Q Exactive HF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, and Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC to offer a phosphorylation quantitative proteomics analysis service package. Simply inform us of your experimental objectives and send your samples, and BiotechPack will handle all subsequent project matters, including protein extraction, protease digestion, phosphorylated peptide enrichment, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

BTP-Phosphorylation Quantitative Proteomics Analysis Workflow

 

Phosphorylation Quantitative Proteomics Analysis Workflow

 

BTP-Phosphorylation Quantitative Proteomics Sample Requirements

1. If you are providing tissue samples, please send them on dry ice; plant tissue samples should be over 200mg, blood samples at least 1ml (ensure plasma uses EDTA anticoagulant), serum 0.5ml, urine 2ml, animal tissue samples at least 1g, cell samples should be 5X10^7 cells, and yeast or microbial samples should have a dry weight of 200mg.
2. If you are providing protein samples, please ensure the total protein amount is above 1mg; regular tissue or cell lysis buffers can be used for protein extraction.
3. Sample transportation: Please use sufficient dry ice for shipping and choose the fastest possible delivery method to minimize the risk of sample degradation during transit.
4. Before the formal experiment, we will test the sample you provide, and the official experiment will begin once the sample passes the quality check.

BTP-Phosphorylation Quantitative Proteomics Research Case Study

This project involved the identification of protein phosphorylation sites in Co-IP samples conducted by a professor at the Institute of Biophysics, Chinese Academy of Sciences at BiotechPack. Part of the results is shown in the figure below:

 

Phosphorylation Quantitative Proteomics Analysis Case Study

 

Bilingual Project Report

In the technical report, BiotechPack will provide you with a detailed bilingual (Chinese and English) report, including:

1. Experimental procedures (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Detailed information on identified phosphorylation sites
4. Mass spectrometry images
5. Raw data

One-stop Service for Phosphorylation Quantitative Proteomics

You only need to place an order and send samples
BiotechPack's one-stop service completes: sample processing - machine analysis - data analysis - project report

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