Cell surface proteins are a special class of proteins. They have very important functions, such as managing cell functions and communicating between the cell and its environment through the transport of metabolites, ions, and other solutes.
Cell surface proteins vary between different cell types. Additionally, even within a specific cell type, they can change under normal or diseased conditions. Therefore, there are many important applications of cell surface proteins, such as distinguishing cell phenotypes and disease states, and research and development for prognosis and therapeutic targets. Cell surfaces present key biomarkers and potential drug targets for various diseases. Currently, about 70% of developed biopharmaceuticals target cell surface proteins, making the study of surface proteins particularly important and a potential pathway for new therapies for various cancers.
Workflow for Cell Surface Proteomics (Mathias Kalxdorf et al., 2017)
Biotech Pack provides professional and comprehensive cell surface proteomics services for the identification and quantification of cell surface proteomes, including the entire process from cell surface protein extraction to protein identification and quantification through mass spectrometry analysis.
Based on biotin affinity purification technology, Biotech Pack has optimized protocols for the purification and enrichment of cell surface proteins, enabling high-quality extraction and enrichment of cell surface proteins for subsequent proteomic analysis. Biotech Pack uses the highest resolution and sensitivity mass spectrometer currently available, Thermo's latest Orbitrap Fusion Lumos, combined with nanoLC-MS/MS for cell surface proteomics analysis. With the aid of powerful bioinformatics, our technicians, who have extensive analysis experience in the field of bioinformatics, can proficiently use various bioinformatics tools to deeply analyze and mine data, enabling the screening of potential biomarkers.
