Shotgun Proteomics

Shotgun proteomics refers to the use of bottom-up proteomics techniques to study all proteins in complex mixtures such as serum, urine, and cell lysates. It combines high-performance liquid chromatography (HPLC) technology and mass spectrometry (MS). The most unique aspect of shotgun proteomics is that it allows for the identification and quantification of multiple proteins with minimal protein separation.

The purpose of shotgun proteome analysis is to identify as many proteins as possible in a sample. Shotgun proteome analysis utilizes shotgun technology for comprehensive protein analysis, where the basic method involves enzymatic digestion of proteins in a mixture, followed by separation of peptides through liquid chromatography (LC), and then identification of peptides using tandem mass spectrometry (MS/MS) to achieve protein identification. Shotgun proteome analysis allows for the identification of all proteins in a sample, capable of simultaneously identifying hundreds to thousands of proteins.

Baitaipike Biotechnology's shotgun proteome analysis service is based on ultra-high resolution mass spectrometry technology, using Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography, enabling comprehensive identification of a large number of proteins in entire cells, tissues, or complex mixed samples.

Shotgun proteome analysis process

Zhu, Y. F. et al. Karolinska Inst. 2018.

Shotgun proteome analysis

Advantages of BTP-Shotgun Proteomics

• Enables large-scale and global protein identification and quantification
• Allows comparative analysis of different samples
• Ideal for discovering and studying protein biomarkers
• Applicable to dynamic proteomics analysis

Applications of BTP-Shotgun Protein Identification Service

Identification and quantification of single proteins
Identification and quantification of proteins in complexes
Identification and quantification of post-translational modifications
Protein interaction analysis

Technical platform

Liquid Chromatography (LC)
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-MS)
Electrospray Ionization Mass Spectrometry (ESI-MS)

Project report in Chinese/English

In the technical report, Baitaipike will provide you with a detailed bilingual technical report in Chinese and English, including:

1. Experimental steps (Chinese and English)
2. Related mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Detailed information on identified proteins

One-stop service for shotgun proteome analysis

You only need to place an order and send the samples
Baitaipike's one-stop service completes: sample processing - on-machine analysis - data analysis - project report