Protein Circular Dichroism Analysis
Asymmetrical secondary structures such as α-helices, β-sheets, and β-turns exist in protein molecules, which result in different absorption of left and right circularly polarized light. After passing through these structures, the light becomes elliptically polarized. This phenomenon is known as the circular dichroism of proteins. Circular dichroism (CD) spectroscopy is an important technique used to analyze the secondary and tertiary structures of optically active molecules in solution, such as proteins and DNA. It is widely applied in the study of the stereostructure, function, and interactions of macromolecules.
BiotaiPack provides professional circular dichroism services for analyzing and determining the spatial configuration of proteins. This technique has the following advantages over traditional methods:
1. Short measurement time, simple, and efficient
2. Requires a minimal amount of sample, can be measured in dilute solutions
3. No molecular weight or size limitations
4. Highly sensitive to changes in secondary and tertiary structures, capable of detecting minor alterations
Norma, J. G. et al. Nat. Protoc. 2006.
Circular dichroism illustrates representative secondary structures of peptides and proteins
Sample requirements:
Protein sample concentration >0.5 mg/mL, purity >90%, and solid protein mass must not be less than 200 µg
Project report in Chinese/English
In the technical report, BiotaiPack will provide you with a detailed bilingual report in Chinese and English, including:
1. Experimental procedures (Chinese/English)
2. Related data (Chinese/English)
Related services:
Circular dichroism analysis (CD)
Protein structure identification
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