Interaction Protein Mass Spectrometry Identification

Interactive Protein Mass Spectrometry Identificationis a method that utilizes mass spectrometry technology to study protein interactions, itseparates target protein complexes through immune(co)precipitation, affinity purification, and other methods;thenuses enzymatic digestion of proteins to produce peptides,utilizesliquid chromatography-mass spectrometry (LC-MS/MS) to analyze peptides; finally, based on mass spectrometry data, combined with bioinformatics tools, it identifies and quantifies interacting proteins. Interactive protein mass spectrometry identification can identify or verify interacting proteins, discover new proteins that interact with target proteins,identify and quantify interacting proteins,and reveal proteinsignaling pathways within organisms, aiding in drug target discovery and biomarker research, providing a basis for drug development.

 

Baitai Pack Biotechnology BTP offers a one-stop interactive protein mass spectrometry identification service based on Thermo Fisher's Orbitrap Exploris 240 mass spectrometry platform combined with Nano-LC, capable of mass spectrometry identification and analysis of IP, Co-IP samples, and GST fusion protein pull-down purified samples. The pre-processing of protein samples for identification strictly follows the article published in Nature Methods: Universal sample preparation method for proteome analysis, ensuring higher peptide coverage in the identification experiment.

 

Baitai PackBiotechnology BTPInteractive Protein Mass Spectrometry IdentificationProcess

1.Protein Extraction:

Use the best methodto extract proteins from cell or tissuesamples, measure proteinpurity andconcentration, to ensure the quality of the extract.

 

2.Acquisition of Interactive Proteins:

According to experimental needsuse immune(co)precipitation, (GST) pull-downorimmunoblottingmethods to enrich target proteins and their interacting proteins.

 

3. Elution and Enrichment:

Elute target protein complexes from binding media, enrichand collect protein samples for subsequent mass spectrometry analysis.

 

4.Protein EnzymaticDigestion:

Usea variety of proteases (such as trypsin) to digest eluted interactive protein group samples, generating peptides.  

 

6.Peptide Separationand Purification:

Usehigh-performanceliquid chromatography(HPLC) orreverse-phase high-performance liquid chromatography(RP-HPLC)technologyto separate and purify enzymatically digested peptides.

 

7.Mass Spectrometry Analysis:

Perform mass spectrometry analysis on peptides using LC-MS/MS to obtain mass spectrometry data.

 

8.Data Processing and Analysis:

Search mass spectrometry data in databases, perform bioinformatics analysis,qualitatively and quantitativelyidentify interactive proteinsmass.

 

OurAdvantages

1.High Sensitivityand High Accuracy:

Ourmass spectrometry technologycanaccuratelydetectinteractiveproteins in complex biological samples, even low-abundanceinteractiveproteins can be identified,with high sensitivity. 

 

2.High Throughput:

Mass spectrometry technology can identify and quantify large numbers of proteins in a short time, saving researchers time and experimental costs.

 

3. Various Types of Protein Interactions:

Our services cover multiple types of protein interactions, including homologous, heterologous, transient, and permanent interactions.

 

4.Relatively Unbiased:

Compared to traditional methods like gene chips and yeast two-hybrid systems, mass spectrometry offers relatively low bias in detecting protein interactions.

 

5. Broad Applicability:

Mass spectrometry can be applied to various biological systems, such as bacteria, yeast, plants, animals, and humans, supporting research in multiple biological fields.

 

6.Rich Structural Information:

Mass spectrometry can provide information on protein post-translational modifications, cleavage, protein-protein interactions, and more, helping researchers gain a deeper understanding of protein functions and regulatory mechanisms.

 

Sample Information

1. Total ProteinExtracts: Can be extracted from cells, tissues, or organisms. It is recommended to provide at least 100 µg of protein to ensure experimental success.

 

2. Enriched Proteins: For the identification of interacting proteins of a specific target protein, you can provide purified samples from IP, Co-IP samples, and GST fusion protein pull-down protein/protein mixtures. It is recommended to provide at least 50 µg of enriched proteins.

 

Bilingual Project Report

In the technical report, Baitai Pack will provide you with a detailed bilingual technical report in both Chinese and English, including:

1. Experimental Steps (in Chinese and English)
2. Related Mass Spectrometry Parameters (in Chinese and English)
3. Details of Interactive Protein Mass Spectrometry Identification
4. Mass Spectrometry Images
5. Raw Data

 

One-Stop Interactive Protein Mass Spectrometry Identification Service

You only need to place an order and send samples
Baitai Pack's one-stop service completes: Sample processing - Instrument analysis - Data analysis - Project report

 

Related Services

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GST Fusion Protein Pull-down Combined with Mass Spectrometry for Protein Interaction Analysis
SILAC Combined with Co-Immunoprecipitation and Mass Spectrometry for Protein Interaction Analysis
Protein Interaction Analysis
Protein Mass Spectrometry Identification