Protein Comprehensive Analysis

Proteins are the main functional components of cells.，Studying proteins allows for a deeper understanding of biological processes, disease occurrence, and drug mechanisms. Proteomics is a systematic approach to studying the expression, function, and interactions of all proteins in a biological sample.This method is also known as shotgun proteomics.analysis.The aim of proteomics is to identify and analyze as many proteins as possible in the sample.By lysing, digesting, and separating protein samples, followed by the detection and identification of peptides using high-resolution mass spectrometry, the proteins present in the sample can be determined. Proteomics enables the simultaneous identification of hundreds or even thousands of proteins in a sample.

BTP Proteomics One-stop Service by Biotyper Biotech is based on ultra-high resolution mass spectrometry. Using Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography, it can comprehensively identify a large number of proteins in entire cells, tissues, or complex mixed samples.

Biotyper Biotech BTPProteomicsGeneral Process

1.SamplePreparation:Collected from biological samplesExtract proteins to be analyzed from biological samples，Concentrate and desalinate。

2.Separation and purification：SeparateExtractedSeparate the complex protein mixture into individual or partially purified proteins。

3.Digestion：Cut proteins into smaller peptide fragments for mass spectrometry analysis。

4.Peptide separation：Further separate the enzymatically digested peptide mixture using high-performance liquid chromatography (HPLC)。

5.Mass spectrometryAnalysis:Obtain peptidemass-to-charge ratioinformation through mass spectrometry analysis。

6.Data processing and identification：Process the data from the mass spectrometry analysis and identify protein sequences, quantify, and determine modifications through database comparison。

7.BioinformaticsAnalysis:Analyze protein function annotation, pathway analysis, protein interaction networks, etc., to reveal changes in biological processes。

 

Comprehensive Proteomic AnalysisTechnical Advantages

1.Discover functionally important but low-abundance proteins

2.Systematic StudyEntire ProteometoReveal the complex regulatory mechanisms in biological processes

3.QuicklyComprehensive identification and quantificationA large number of proteins in entire cells, tissues, or complex mixed samples

4.Discovery and study of protein biomarkers

Proteome-wide analysisTechnical application

1.Compare the differences in protein expression between normal and diseased cells，ScreeningDrug targets and biomarkers

2.Construct a proteomics database to provide resources for subsequent research

3.Identification and Quantification of Intracellular ProteinsRevealing Molecular Mechanisms of Biological Systems Under Different Conditions

About the Sample

Sample Type	Tissue	Blood	Urine and Saliva	Cerebrospinal Fluid
Sample Quantity	50-100mg	10-100ml	1-10mL	10-100mL

MediumEnglish Project Report

In the technical report, BioTay Parker will provide you with a detailed bilingual technical report in both Chinese and English, including:

1.Experimental Procedures (Chinese and English)

2.Related Mass Spectrometry Parameters (Chinese and English)

3.Comprehensive Protein Spectrum AnalysisDetailed Information

4.Mass Spectrometry Images

5.Raw Data

Comprehensive Protein Spectrum AnalysisOne-Stop Service

You only need to place an order and send the samples
BaiTaiPike provides a one-stop service: Sample processing - Instrument analysis - Data analysis - Project report

Related Services

Shotgun Proteomics Identification

Protein Mass Spectrometry Identification

Pull-down Target Protein Mass Spectrometry Identification

Peptide Mass Spectrometry Identification

Quantitative Proteomics Analysis