Plant Proteomics
Plant Proteomicsis a branch of the field of proteomics, aimed atstudyingthe proteins of plantscomposition, structure, function, interactions, and regulatory mechanismsetc., with research methods similar to proteomics, involving core technologies such as protein separation, purification, identification, functional annotation, interaction studies, and expression regulation studies.Research in plant proteomicsnot only provides a material basis for the principles of plant growth, development, and stress adaptation,but also offers theoretical foundations and solutions for improving crop stress resistance and quality. By comparing proteomes under different growth conditions, different plant varieties, and between normal and stressed individuals, we can identify certain "specific protein molecules" that may serve as molecular targets for genetic improvement and biotechnological strategies, or provide molecular markers for the impact of environmental changes on plant growth.
Biotech Company BTPPlant ProteomicsService Content
1.Protein Identification and Functional Annotation
Using high-resolution mass spectrometry (Thermo Fisher's Q Exactive and Orbitrap), combined with bioinformatics databases and software, we identify the detected proteins and provide detailed functional annotations.
2.Proteome Quantitative Analysis
Providing both labeled (iTRAQ, TMT, SILAC) and label-free methods for quantitative protein analysis.
3.Protein Interaction Studies
Using methods such as immunoprecipitation (IP), co-immunoprecipitation (Co-IP), or affinity purification mass spectrometry (AP-MS), we study interactions between proteins of interest and others, followed by mass spectrometry identification of proteins/protein complexes in IP, Co-IP samples, and GST fusion protein pull-down purified samples using LC-MS/MS.
4.Post-Translational Modification (PTM) Analysis
Providing identification of phosphorylation/glycosylation/ubiquitination/acetylation/methylation/disulfide bond/nitrosylation post-translational modifications, including modification sites and quantitative analysis.
5.Protein Expression Differential Analysis
By comparing plant proteome data under different treatment conditions,conductprotein expression differentialstatisticalanalysis(Venn diagram, Volcano plot) and clustering analysis (hierarchical clustering, K-means clustering).
Plant ProteomicsTechnologyofApplication
1.Gene Research
By analyzing differences in plant protein expression, provide information support for gene function prediction, gene regulatory network researchandplant physiological ecology research.
2.Agricultural Improvement
Study the protein components and metabolic pathways in plant products,canprovideagricultural biotechnologyreferencesupport.
3.Ecological Research
Understanding the biological mechanisms of plant adaptability changes can aid in ecological protection and biodiversity research.
4.Plant Disease Diagnosis
Study molecular mechanisms of disease,providing important information for disease diagnosis and control.
About Samples
We can accept various types of plant samples, including but not limited to:
1. Fresh tissue: such as leaves, roots, stems, flowers, and seeds; at least 100 mg.
2. Dried tissue: plant tissue that has been freeze-dried or oven-dried; at least 50 mg.
3. Cells: plant cell suspensions or cell lysates; at least 1x10^7 cells.
4. Protein extracts: extracted protein samples, at least 100 μg, with a concentration no less than 1 μg/μl.
Chinese/English Project Report
In the technical report, Biotech Company provides you with a detailed bilingual version of the technical report, which includes:
1. Experimental procedures (Chinese & English)
2. Relevant mass spectrometry parameters (Chinese & English)
3.Plant ProteomicsAnalysis Details
4. Mass spectrometry images
5. Raw data
Plant ProteomicsOne-Stop Analysis Service
You only need to place an order and send samples
Biotech Company provides one-stop service: sample processing - on-machine analysis - data analysis - project report
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