HPLC Detection of Protein Purity

HPLC (High Performance Liquid Chromatography), also known as high-efficiency liquid chromatography or high-pressure liquid chromatography, is a new analytical technology developed based on liquid chromatography technology in the last 20 years. It is used for the separation, identification, and quantification of various components in non-volatile organic compounds.

The principle of HPLC is basically the same as that of liquid chromatography. The difference lies in the fact that HPLC applies pressure to the mixture through a high-pressure pump, allowing it to quickly pass through the chromatographic column filled with solid adsorbent, thereby enhancing the separation speed. Additionally, the use of high-sensitivity detectors and highly efficient microparticle stationary phases greatly improves the separation efficiency and sensitivity of HPLC.

HPLCProtein detectionPurity

Protein purity is a crucial indicator in protein analysis. Proteins isolated in experiments often contain some impurity proteins or reagents used in extraction steps, affecting the smooth progress of subsequent experiments. Therefore, it is necessary to purify them and detect the results of purification. Protein purity determination can evaluate whether the protein contains impurities and the content of impurities, which is a vital step for subsequent protein research.

HPLC detection of protein purityThe principle is to add the protein sample into a chromatographic column filled with solid adsorbent. Different protein samples interact differently with the adsorbent material, resulting in different elution speeds, allowing the protein samples to be separated. The separated proteins are then analyzed through a detection system, and chromatographic data combined with the corresponding database are analyzed to identify the type and content of the proteins.

Biotech company Baitai Parker provides protein qualitative, quantitative, and purity testing services based on HPLC and mass spectrometry. These services can be used for the separation and determination of various protein/peptide samples, meeting the needs for purity analysis of various protein/peptide samples. For a free consultation, please call 183-1097-1257.

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