Immunoprecipitation Mass Spectrometry

Immunoprecipitation (IP) is a technique developed based on immunological principles and traditional affinity purification methods to detect, isolate, and purify proteins. Proteins obtained through immunoprecipitation can be further identified by mass spectrometry analysis, known as IP mass spectrometry analysis. IP mass spectrometry analysis has been widely used in studying protein types, quantities, expression changes, and protein-protein interactions.

The immunoprecipitation mass spectrometry technique first obtains the target protein (antigen) through immunoprecipitation, then digests the target protein into peptide fragments for mass spectrometry analysis. Finally, the mass spectrometry data is combined with bioinformatics analysis, enabling qualitative and quantitative analysis of the target protein.

Biotech company Biotai-Pike offers a service package using Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC for immunoprecipitation mass spectrometry. This service can perform subsequent qualitative and quantitative identification of IP samples and also provide identification services for protein/protein mixtures in Co-IP samples and GST fusion protein pull-down samples. Free consultation is available.

Related services:
CO-IP immunoprecipitation protein interaction analysis
GST pull-down protein interaction analysis
Protein interaction analysis combining SILAC and co-immunoprecipitation with mass spectrometry
Protein interaction mass spectrometry analysis
Mass spectrometry identification service for protein spots, strips, IP, CO-IP, pull-down samples
Pull-down target protein mass spectrometry identification
Protein interaction analysis