Protein Reverse Phase Chromatography Mobile Phase

Liquid chromatography can be categorized into liquid-solid chromatography, liquid-liquid partition chromatography, ion-exchange chromatography, size-exclusion chromatography, affinity chromatography, etc., based on different separation mechanisms. Reversed-phase liquid chromatography (RPLC) is a type of liquid-liquid chromatography technique where the mobile and stationary phases are two immiscible liquids with opposite polarities. This method involves coating specific liquid substances on the carrier surface or chemically bonding them to form a liquid stationary phase, allowing separation based on the differing solubility of components in the mobile and stationary phases. It is widely used in the separation and analysis of compounds such as proteins and peptides, as well as in purity identification.

The characteristic of reversed-phase chromatography is that the stationary phase has a weaker polarity compared to the mobile phase. Conversely, in normal-phase chromatography, the stationary phase is more polar than the mobile phase. RPLC uses non-polar substances as the stationary phase, such as C₁₈, C₈, etc. The mobile phase is a polar liquid such as water or a buffer solution. To adjust the retention time of sample substances and control their dissociation during the analysis process, organic solvents like methanol, acetonitrile, acetone, isopropanol, tetrahydrofuran, etc., which are miscible with water, are often added to the mobile phase.

Biotech company Biotage offers high-resolution chromatography platforms, providing fast and efficientprotein reversed-phase chromatography analysisservice packages, which can be used for the separation and determination of various protein/peptide samples, meeting the needs for purity analysis of diverse protein/peptide samples. Feel free to inquire for more information.

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