What is SEC for Antibody-Drug Conjugates

Antibody-drug conjugates (ADCs) are a new type of biopharmaceutical that serve as powerful tools in the treatment of tumors and cancers. They are composed of monoclonal antibodies and small molecule cytotoxic drugs, combining the high specificity of antibodies with the potent killing ability of small molecule drugs. This targeted attack on tumor cells improves the targeting specificity of cancer drugs while reducing their side effects, making ADCs of significant importance in clinical treatment.

Size Exclusion Chromatography (SEC), also known as gel filtration chromatography or gel permeation chromatography, is used to characterize substances based on differences in their volume and mass, leading to differences in elution speed and time in the chromatographic column. It provides various characterizations such as molecular weight, molecular weight distribution, and purity. SEC is widely used in the characterization of volumetric isomers in protein biopharmaceuticals. ADC SEC utilizes size exclusion chromatography to analyze ADC drugs and characterize their critical quality attributes, such as volumetric isomers. Protein biopharmaceuticals can form high molecular weight (HMW) aggregates and low molecular weight (LMW) fragments during development, production, transport, and storage. These volumetric isomers may lead to immunogenic reactions, pharmacokinetic or potency differences in protein drugs, necessitating characterization of these critical quality attributes (CQAs). SEC is the standard technique for analyzing volumetric isomers of ADC drugs.

Biotech company Biocytogen offers one-stop SEC analysis services for antibody-drug conjugates using high-resolution chromatography technology. A variety of chromatographic columns with different performance characteristics are available to meet various testing needs, and customized testing solutions are provided free of charge. Please contact us for more information!

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