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[New Product Launch] High-Throughput Gene Knockout Service

New hot products, year-end special offer!

▶No waiting, cell lysate delivered in 2 weeks

▶Pay after successful verification, worry-free

▶Custom knockout cell pool (KO pool), knockout efficiency ≥ 70%

▶Significant discounts on monoclonal and overexpression as well

▶Special discount of 5888 RMB for multi-omics project clients

Event time: 2024.1.1-2024.2.29

Event project: High-throughput gene knockout service

BiotechPack Scientific Co., Ltd. (BTP) uses an optimized CRISPR/Cas9 system to provide high-throughput gene knockout services. Our human genome-wide gene knockout array library covers the entire human genome, and the whole genome array knockout library has been fully validated in primary human cells through high-content screening.Over 80% of targets achieve a knockout efficiency of over 70%.

BTP is committed to providing you with the simplest and most user-friendly high-throughput CRISPR gene knockout services, including viral packaging, plasmid preparation, and Cas9 concentration testing, addressing key issues such as which cells to knockout, feasibility of knockout, how to knockout, and knockout efficiency.Capable of single/multiple gene knockouts, frameshift mutations, and large sequence deletions. You only need to provide the gene name and target cells; BiotechPack Scientific Co., Ltd. handles all subsequent project tasks, including project evaluation (whether the target gene is expressed in the target cells, whether it is an essential gene), KO experiments, KO cell validation, storage of KO cells, and delivery of live KO cells.

Service Advantages

1. Strong sample applicability

Applicable to human, mouse cell lines, primary cells, immune cells, and iPS cells, etc.

2. Gene necessity and expression level analysis

Before conducting gene knockout experiments, we analyze the necessity and expression levels of genes in different cells based on bioinformatics and proteomics data to ensure the feasibility of the experiment and reduce experimental risks.

3. No need to design and validate gRNA from scratch

We have completed the validation of the entire human genome gRNA and PCR sequencing validation for drug-related targets, eliminating the need for pre-experimental gRNA validation for customer targets, greatly shortening the project cycle.

4. High-throughput and high efficiency

We use an optimized CRISPR/Cas9 system for high-throughput gene knockout, with multiple sgRNA/target gene designs ensuring high knockout efficiency. Over 80% of target knockout efficiencies exceed 70%, and high-efficiency KO pools can be provided within a short period (within 10 days), meeting the needs of most applications.

5. Dual validation of knockout efficiency

After gene knockout, we use Sanger sequencing and advanced proteomics techniques, which are the preferred methods for antibody KO verification in WB experiments, to conduct dual validation of knockout efficiency, ensuring data authenticity.

Welcome to visit the BiotechPack homepage, contact us for more service details!

Beijing BiotechPack Scientific Co., Ltd. (BTP) was established in 2015 and specializes in biological product characterization based on mass spectrometry, macromolecule analysis (including proteins, peptides, and metabolites), and small molecule detection services.

The company implements the ISO9001 quality control system and professionally provides CRO testing and analysis services based on mass spectrometry;
Accredited by the national CNAS laboratory, providing drug quality research services that comply with global pharmaceutical regulations;
Business scope covers proteomics, peptidomics, metabolomics, biopharmaceutical characterization, single-cell analysis, single-cell mass spectrometry flow, bioinformatics cloud analysis, and multi-omics mass spectrometry integration analysis, etc.;
7 major quality control testing platforms to meet your one-stop service needs;
Serving 3000+ enterprises and the choice of 10,000+ customers;
Committed to providing you with high-quality bio-mass spectrometry analysis services!