Protein Identification Service Discount is Coming -- April

Baite Parker Bio uses high-resolution mass spectrometry platform technology, combined with Nano-LC high-throughput liquid chromatography technology, to achieve efficient and precise identification/determination of proteins and protein molecular weights in samples such as protein extracts, SDS-PAGE protein bands, 2D protein spots. For the identification of single proteins and simple protein mixtures, we guarantee a 100% identification rate, otherwise no fee will be charged.

Event Content

Service Items:Protein Molecular Weight Determination,Protein Spot, Gel Strip, IP Sample Protein Identification,Pull-down Target Protein Identification

Event Duration: April 1, 2019 -- April 15, 2019

Event Details:

1. During the event period, order the above items and enjoy a 12% discount
2. While ordering the service, successfully refer a new customer and both will enjoy a 20% discount

Event Rules:

1. This discount event cannot be combined with other promotions;
2. The final interpretation right of this event belongs to Beijing Baite Parker Biological Technology Co., Ltd.

Protein Identification Process

Based on the Shotgun method: Protein bands or protein solutions are enzymatically digested into peptide mixtures, which are then separated by high-performance liquid chromatography into several simpler components. These are analyzed in a high-resolution mass spectrometer. The search software compares the secondary mass spectrometry information with the corresponding database, combines matching scores and mismatch filtering, and determines the exact sequence of the peptides, thus assembling the complete sequence of each protein to achieve protein identification.

Pull-down Target Protein Identification Process: Express and purify bait proteins labeled with GST, polyHis, or Biotin, use immobilized affinity ligands to stably bind bait proteins to solid-phase matrices, then incubate the bait proteins with the cell lysate to be tested. During this process, bait proteins interact and bind with target proteins. By washing away non-specifically bound contaminant proteins, target proteins interacting with bait proteins can be obtained. Then, use mass spectrometry analysis to identify the target proteins.