Protein Identification Service Event - April [2020]

Baite Parker Biotechnology utilizes ultra-high resolution mass spectrometry technology, based on Thermo Fisher's Q Exactive mass spectrometer, LTQ Orbitrap Elite mass spectrometer, Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer, and AB SCIEX's 6500 Q TRAP mass spectrometer, combined with Nano-LC liquid chromatography technology. This allows for efficient and precise identification of proteins in samples such as protein extracts, SDS-PAGE protein bands, 2D protein gel spots, pull-downs, and co-IP. We guarantee a 100% identification rate for single proteins and simple protein mixtures, otherwise, no fee will be charged.

Event Details

Event Projects:Protein Molecular Weight Determination, Protein Separation Based on SDS-PAGE,Protein Identification from Gel Spots/Bands/IP Samples,Pull-down Target Protein Mass Spectrometry Identification,Peptide Identification,Native Mass Spectrometry Analysis (Native MS)

Event Date: April 23, 2020 - April 28, 2020

Discount Details: Enjoy a 12% discount with your order

Note: This offer cannot be combined with other promotions

Identification Process

Sample Pre-treatment: Proteins are reduced and alkylated, then digested with highly specific proteases to obtain peptide fragments

Upstream Analysis: Peptides are separated via nano-flow chromatography combined with high-resolution mass spectrometry, collected with the highest sensitivity ultra-high resolution mass spectrometer

Database Search: Analyzes thousands of mass spectra in conjunction with species-specific protein databases (using the Uniprot database, updated monthly to ensure access to the latest data) to identify proteins in the sample

Report Issuance: The identification report will include detailed sample pre-treatment steps, chromatography-mass spectrometry platform parameters, and the identified protein and peptide lists. Raw mass spectrometry data will be provided via cloud storage

Sample Preparation

1D SDS PAGE /2D PAGE Gel Samples

1. Samples stained with Coomassie Blue, SYPRO Ruby, and Silver stain can all be used for protein identification experiments;
2. Silver-stained samples may be incompatible with subsequent mass spectrometry analysis. We recommend using the following products or experimental steps for silver staining:

ProteoSilver Plus, Sigma (Product # PROTSIL1 or PROTSIL2)

Dodeca Silver Stain, BioRad (Product # 161-0481 or 161-0480)
3. Do not decolorize silver-stained samples.

Liquid Samples (In solution format)

1. If the eluted sample needs to be run on a gel, prepare the sample according to the 1D SDS PAGE sample preparation requirements;
2. If the protein amount in the sample is >10ug, there are no buffer system requirements for the sample;
3. If the protein amount in the sample is <10ug, minimize the use of surfactants such as SDS during sample preparation, and reduce salt concentration;
4. When submitting samples, please also provide the buffer system of the sample and the estimated protein content in the sample.

Notes

1. Wear gloves if you need to cut the gel;
2. Keep the blade for cutting the gel clean;
3. Cut the gel strip as precisely as possible, as extra gel outside of the strip may generate background noise;
4. Avoid keratin contamination, which generally originates from sample preparation, gel running, gel cutting processes, or even laboratory air. Try to conduct experiments in a clean environment.

Contact Information

Scientific Service Phone: 010-67869385

Mobile/WeChat: 152-0137-7680

QQ: 3320745850

Email: info@biotech-pack.com

Address: No. 88, Kechuang Sixth Street, Beijing Economic-Technological Development Area