Comprehensive Analysis of Post-Translational Modifications

In life activities, post-translational modifications (PTMs) of proteins are critical mechanisms for regulating protein function, stability, localization, and interactions. They are extensively involved in various life processes, including cell signal transduction, metabolic regulation, immune response, cell cycle, and disease progression. With the development of omics technologies, researchers are increasingly focused on the synergistic effects of PTMs in complex biological systems. However, due to the diversity of modification types, low abundance, and rapid dynamic changes, traditional detection methods often face challenges such as insufficient sensitivity, inaccurate qualitative analysis, and difficulty in modification site resolution.

 

Post-translational modification refers to a series of covalent modifications that occur on amino acid residues after protein translation is completed, including but not limited to phosphorylation, acetylation, methylation, glycosylation, and ubiquitination. Common research methods include mass spectrometry analysis, especially when combined with high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) technology, which can achieve high-throughput identification, localization, and quantification of modified peptides, making it the mainstream technical approach for current PTM research.

 

Relying on an advanced mass spectrometry platform and a mature sample preparation system, Biotai Parker offers multi-type post-translational modification omics analysis services, covering mainstream modification types such as phosphorylation, acetylation, methylation, ubiquitination, and glycosylation. We provide researchers with multidimensional information support, including precise identification of modification sites, quantitative analysis of modification levels, and signal pathway enrichment, through high-sensitivity enrichment strategies and professional data analysis processes. This helps explore protein function regulation mechanisms and accelerates research translation from phenotype to mechanism. The types of post-translational modification analysis services we offer include but are not limited to the following aspects:

 

Types of Post-Translational Modifications
Phosphorylation	Acetylation
Methylation	Ubiquitination
SUMOylation	Glycosylation
Hydroxylation	Carboxylation
Nitration	S-nitrosylation
Carbonylation	Polyglutamylation
Lipidation	Amidation
Oxidation	Lactylation
Prenylation	Lipovlation
Cysteinylation	Arginylation
Selenylation	Adenylylation
S-Glutathionylation	Carbamylation

 

Technical Platform

High-throughput omics analysis of post-translational modifications requires extremely high demands on instrument resolution, sensitivity, and data accuracy. Biotai Parker Biotechnology relies on multiple high-resolution mass spectrometry platforms, combined with advanced liquid chromatography (LC) and specialized enrichment technologies, to achieve precise identification, site localization, and quantitative analysis of various modification types. This is suitable for different sample types and research needs, ensuring the breadth and depth of data. The types of instruments we use mainly include the following:

 

 

Analysis Process

 

Experimental Roadmap for Post-Translational Modifications

 

Flowchart of Post-Translational Modification Analysis

 

Characteristics of Common Types of Post-Translational Modifications

 

Modification Types	Modification Sites	Enrichment Methods
Phosphorylation	Ser、Thr、Tyr	TiO₂ Enrichment, IMAC Enrichment
Acetylation	Lys	Anti-acetyl lysine antibody
Methylation	Lys、Arg	Anti-methylation antibody enrichment
Ubiquitination	Lys	K-E-GG antibody enrichment
SUMOylation	Lys	Anti-SUMO antibody enrichment
Glycosylation	Asn(N-type), Ser/Thr(O-type)	Lectin、HILIC
Lactylation	Lys	Anti-lactyl lysine antibody enrichment

 

Sample Submission Requirements

 

Modification Type / Sample Type	Serum/Plasma	Animal Tissue	Plant Tissue	Microorganism	Protein Extracts	Cells
Phosphorylation	50 μL	100 mg	2 g	500 μL	2 mg	2×10⁷
Glycosylation
Acetylation	100 μL	200 mg	10 g	500 μL	5 mg	2×10⁸
Ubiquitination
SUMOylation
Lactylation
Methylation

Service Advantages

• Equipped with high-resolution mass spectrometry platform to ensure modification detection sensitivity

• Multiple enrichment strategies to enhance specificity and coverage

• Supports various sample types to meet different experimental needs

• Experienced team ensures data accuracy and reliability

• Provides one-stop analysis services to accelerate research progress

• Standardized processes ensure stable and highly reproducible results

• Supports customized solutions to meet diverse research goals

 

Application Fields

• Biomedical Research — Tumor Biomarker Screening, Disease Mechanism Analysis

• Drug Development — Drug Mechanism Validation, Target Modification State Analysis

• Cell Biology — Cell Cycle Regulation, Transcriptional Regulation Mechanism Analysis

• Epigenetics — Histone Modification Profiling, Gene Expression Regulation Research

• Neuroscience Research — Neurotransmitter Regulation, Neurodegenerative Disease Mechanism Exploration

 

Data Analysis

• PCA、PLS-DA/OPLS-DA、Volcano Plot、Heatmap 

• GO Enrichment Analysis

• KEGG Pathway Analysis

• Cytoscape Network Analysis

• STRING Protein Interaction Analysis

• Pathway Enrichment Diagram

• Enrichment Map

 

Biotech Pack Biotech — Characterization of Biological Products, High-quality Multi-omics Mass Spectrometry Detection Service Provider

 

Related Services:

Post-translational Modification Proteomics Analysis