Protein Cross-Linking Degree Determination

Protein cross-linking is a method that combines the use of specific protein cross-linkers and mass spectrometry (MS) detection to reveal the structural characteristics of proteins and interacting protein complexes. This method has facilitated the development of established methods and bioinformatics tools in protein interaction analysis. Protein cross-linking degree refers to the average relative molecular mass between two adjacent cross-linking sites after protein cross-linking. It is a key indicator for characterizing the degree of protein cross-linking and can be measured using methods such as swelling equilibrium and dynamic torsion oscillation. By measuring the degree of protein cross-linking, it can be used to evaluate the cross-linking degree and effect of protein cross-linkers. Achieving a high degree of protein cross-linking remains one of the significant challenges.

Biotech BioThermo Fisher's Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC technology providescross-linking method protein interaction analysisservices, including in vivo cross-linking, in vitro cross-linking, chemical cross-linking, and photo-cross-linking, to resolve spatial structures of proteins, transient protein interactions in vivo, and stable interactions. You only need to inform us of your experimental purpose and send in your samples, and we will take care of all subsequent matters, including cell culture, cell labeling, protein extraction, antibody IP, efficiency testing, protein digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.

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