De novo protein sequencing service

De novo protein sequencing service refers to an analytical method that directly deciphers the primary structure (amino acid sequence) of proteins using mass spectrometry without relying on any known reference sequence. Compared to traditional protein identification methods that depend on database matching, de novo protein sequencing is more suitable for research scenarios involving newly discovered proteins, unknown modified proteins, antibody variable regions, and non-model organisms. It is one of the indispensable key technologies in modern proteomics and biopharmaceutical research and development. Through this service, researchers can obtain protein sequence information without a reference sequence, supporting subsequent functional research, structure modeling, antibody development, or biomarker screening. De novo protein sequencing service has been widely applied in basic research, drug development, vaccine design, and other fields as a powerful tool for promoting bioinformatics innovation and precise molecular recognition. Currently, de novo protein sequencing service is integrating with other omics technologies to drive the development of multidimensional protein research. For example, comparison with translatomics (Ribo-seq) data can reveal differences between translation products and actual expressed proteins; combining with modification analyses such as phosphorylation and glycosylation helps to comprehensively understand the functional state of proteins; in structural biology, de novo protein sequencing can provide precise sequence basis for cryo-electron microscopy or X-ray crystallography, assisting in the atomic resolution structure analysis. Especially in protein research where the structure is unclear or the sequence cannot be determined by traditional methods, de novo protein sequencing service demonstrates an irreplaceable strategic significance.

 

The core technology of de novo protein sequencing service lies in mass spectrometry analysis. Using high-resolution mass spectrometers (such as Orbitrap or TOF-MS), the peptide ions obtained after enzyme digestion of the target protein are subjected to MS/MS (tandem mass spectrometry) analysis, recording their fragmentation spectra and using algorithms to deduce the amino acid sequence of each peptide. Unlike database-dependent methods, de novo protein sequencing service constructs sequences directly based on the mass differences of fragment ions, without relying on any existing protein database. This capability is particularly important for proteins of unknown origin, with insufficient database coverage, or containing numerous mutations and modifications. For example, in scenarios like polyclonal antibodies, traditional Chinese medicine proteomics, and natural toxin analysis, this technology is the only reliable means to obtain accurate information.

 

The application advantage of de novo protein sequencing service lies in its powerful capability to analyze unknown sequences. Conventional database search methods can only identify known sequences or proteins similar to them, and when the target protein contains variant sites, post-translational modifications, or comes from species not covered by the database, the identification accuracy is greatly reduced. De novo protein sequencing service circumvents this limitation, relying on high-quality fragment spectra and optimized algorithms to reconstruct sequence information, enabling the identification of novel proteins, variant peptides, non-standard residues, and isomers. This feature is particularly crucial in antibody research and development, especially in steps such as monoclonal antibody reconstruction, bispecific antibody screening, and variant site identification, where this technology can directly obtain the variable region sequences of light and heavy chains, aiding in the expression verification and structural optimization of antibodies.

 

Bioytec Biotechnologies specializes in mass spectrometry analysis and proteomics research, offering high-quality, customized protein sequencing services suitable for various project needs such as antibody sequence analysis, novel protein identification, and modification characterization.

 

Bioytec Biotechnologies - A premier service provider for biological product characterization and multi-omics mass spectrometry detection.

 

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Protein sequencing