Auxin Amino Acid Mass Spectrometry Detection Principle

The principle of mass spectrometry detection of auxins and amino acids is based on identifying and quantifying these molecules by their mass-to-charge ratio (m/z). During mass spectrometry analysis, auxin and amino acid samples must first undergo appropriate pretreatment and ionization. Pretreatment includes extraction, purification, and possible derivatization to make the samples suitable for mass spectrometry analysis. Ionization is a crucial step where molecules in the sample are converted into ions. Common ionization methods include Electrospray Ionization (ESI) and Matrix-Assisted Laser Desorption Ionization (MALDI). After ionization, these charged particles are introduced into the mass spectrometer, where they are separated according to their mass-to-charge ratio. Finally, specific signal peaks on the mass spectrum (representing different mass-to-charge ratios) are used to identify and quantify the auxins and amino acids in the sample.

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