Comparison of Olink Proteomics Analysis Platforms: Explore vs. Explore HT
In multi-proteomics research,Olinkthe platform, with its high throughput, low sample requirements, and excellent quantitative performance, has become a mainstream tool in precision medicine, biomarker discovery, and clinical translational research. Olink currently offers two high-throughput analysis platforms: Explore and Explore HT, both based on Olink's unique Proximity Extension Assay (PEA) technology, but they differ in throughput, detection panels, sensitivity, and application scenarios. This article systematically compares the core features of these two generations of platforms to help researchers choose the most suitable solution.
I. Overview of Olink Platform Core Technology
1. Principles of PEA Technology
(1) Specific binding of target proteins by dual antibody probes to form proximity complexes;
(2) Complexes trigger oligonucleotide extension and DNA amplification;
(3) Quantitative analysis achieved using NGS or qPCR readout.
2. Main Advantages of Olink
(1) Single reaction can simultaneously detect hundreds to thousands of proteins;
(2) Only 1-3 μL of plasma/serum is needed per analysis;
(3) High reproducibility across batches (median CV<10%);
(4) Particularly suitable for biomarker discovery, longitudinal cohort studies, and large-scale clinical sample detection.
II. Explore vs. Explore HT: Parameter Comparison
1. Throughput and Detection Content
(1) Explore Platform
① The Explore platform covers approximately 3,000+ human proteins (inflammation, oncology, cardiovascular, neurological, and metabolic pathways);
② The Explore platform can process 96-384 samples per run;
③ The Explore platform uses NGS for detection readout.
(2) Explore HT Platform
① The protein coverage of the Explore HT platform is similar to Explore, but it employs chip-based and automated processes;
② The Explore HT platform can handle thousands of samples per batch, suitable for large-scale cohort or clinical trials;
③ The detection speed of the Explore HT platform is about 2-3 times faster than Explore.
2. Sensitivity and Dynamic Range
(1) Both can detect proteins at fg/mL levels, with a dynamic range of about 10 orders of magnitude;
(2) Explore HT is optimized for detection accuracy and batch consistency of low-abundance proteins, making it more suitable for low-signal biomarker research.
3. Sample Requirements and Applicable Scenarios
(1) Both platforms require only 1-3 μL of sample and support serum, plasma, and some body fluids;
(2) Explore is suitable for exploratory research and moderate sample size mechanism studies;
(3) Explore HT is more suitable for large-scale cohort studies, clinical translation, and drug development.
III. How to Choose the Right Proteomics Research Platform
1. Core Decision Factors
(1) Sample size: Explore is recommended for up to a few hundred cases, HT is recommended for thousands;
(2) Research stage: Explore is suitable for early exploration and mechanism research; HT is selected for validation or clinically related research;
(3) Budget and timeline: HT has a lower per-sample cost and shorter overall cycle.
2. Service Support from Biotye BioTech
(1) Provide customized solutions based on Explore and Explore HT;
(2) Support experimental design, quality control, and high-quality data delivery;
(3) Integrate bioinformatics analysis, delivering results directly usable for publications and project applications.
Under the premise of consistent technical principles, the Olink Explore and Explore HT platforms offer flexible solutions for different research stages and scales. Biotye BioTech combines advanced Olink detection capabilities with comprehensive data analysis support to help research teams efficiently conduct biomarker discovery, mechanism studies, and clinical validation, accelerating the transformation of results.
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