Can ultrasonic cell disruption be used when cells are lysed after using crosslinkers?
When lysing cells after using crosslinking agents, sonication can be used as a lysis method, but it should be used with caution, as the presence of crosslinking agents may affect the effectiveness of sonication.
Usage Recommendations:
1. Sonication Intensity:When performing sonication after using crosslinking agents, it is best to choose a lower sonication intensity and optimize the duration. High-intensity sonication may lead to the breakdown of crosslinking agents or cause non-specific damage.
2. Temperature Control:Temperature may rise during sonication, so it's recommended to use a cooling system (such as an ice bath) to control the temperature and reduce thermal damage, preventing the destruction of crosslinked structures.
3. Optimization of Lysis Conditions:If cells are difficult to lyse after treatment with crosslinking agents, consider using other lysis methods (such as freeze grinding or enzymatic digestion) or combining them with sonication.
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