How long can short-chain fatty acids be stored at -80°C?

Short-chain fatty acids (SCFAs), such as acetic acid, propionic acid, and butyric acid, are relatively stable when stored at -80°C. According to existing literature and laboratory experience, SCFAs can be preserved at -80°C for at least 6 months to 1 year, during which their concentration and structure remain mostly stable, especially under the following conditions:

 

Key factors for stable preservation:

1. Sample matrix: SCFAs can be found in plasma, serum, fecal extracts, and cell culture supernatants. Different matrices may contain enzymes or microbial activity, so inactivation or the addition of antimicrobial agents is necessary before processing.

2. Pretreatment: Rapid freezing after sampling or the addition of acid (such as phosphoric acid or hydrochloric acid) to inhibit microbial metabolism and enzyme activity can significantly extend the stability of SCFAs.

3. Sealed and protected from light: Due to their volatility, SCFAs should be stored in well-sealed, low adsorption tubes (such as EP tubes or glass vials) and kept away from light.

4. pH adjustment: SCFAs are more stable in an acidic environment (such as pH < 3), making them suitable for preservation after acid addition.

5. Freeze-thaw cycles: Avoid repeated freeze-thaw cycles, and it is recommended to store in aliquots.

 

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