How long can a protein sample with loading buffer be stored at -20°C after boiling?
After adding loading buffer to the protein samples and boiling for denaturation, it is generally recommended to store them at -20°C for short-term (no more than 1 month). For long-term storage (more than 1 month), it is advisable to transfer them to -80°C.
The following are key considerations:
1. Degree of Denaturation and Stability:After treatment with SDS and β-ME or DTT and boiling for denaturation, the proteins no longer retain their native conformation and theoretically should not undergo significant degradation. However, repeated freeze-thaw cycles can lead to aggregation or precipitation.
2. Number of Freeze-Thaw Cycles:It is recommended to aliquot and store samples to avoid repeated freeze-thaw cycles, which can cause sample concentration or uneven denaturation.
3. Purpose of Storage:
-
If the samples are to be used for operations like Western blotting or electrophoresis in the short term, storing at -20°C for 1-2 weeks is generally acceptable.
-
If the samples are rare or need to be reused, they should be transferred to -80°C and repeated freeze-thaw cycles should be minimized.
Conclusion: Protein samples with loading buffer and boiled can be stored at -20°C for 1-2 weeks, not exceeding 1 month. For longer-term storage or if the samples are critical, they should be stored at -80°C, avoiding repeated freeze-thaw cycles.
Biotree Biotech--A leading service provider in bioproduct characterization and multi-omics mass spectrometry analysis
Related Services:
How to order?
