Mouse serum samples, what is the typical required volume in microliters for each tube in routine detection? How many samples are needed for each of the three experimental groups?

If the specific type of 'detection' is not explicitly mentioned (such as LC-MS metabolomics, ELISA, Western blotting, clinical biochemistry, transcriptomics, etc.), different platforms and analysis content have significant differences in serum volume and sample quantity requirements. Based on conventional experimental experience, the following are basic requirements for serum volume and sample quantity in mice for several typical detections, which can be used as a reference:

 

I. Serum Volume Required per Tube (Estimated by Conventional Experiments)

 

Type of Experiment	Volume Required (μL/sample)	Remarks
LC-MS Metabolomics	100–200 μL	Consider loss due to freeze-drying/pre-treatment
ELISA	50–100 μL	Depends on kit sensitivity
Western Blotting (WB)	50–100 μL	If extracting protein from serum, recovery rate is low
Clinical Biochemical Indicator Measurement	100–200 μL	Different indicators have different requirements
Multi-omics Integration Analysis	200 μL or more	Multi-platform collaboration requires multiple sample tubes

 

II. Sample Quantity per Group (Statistical Recommendation)

1. Basic Scientific Research Exploration Stage: It is recommended that each group has n ≥ 6, which can be used for preliminary trend judgment.

2. High-throughput Omics Research such as Metabolomics, Proteomics: Recommended n ≥ 8–10 per group to enhance statistical power after multiple comparisons.

3. Preclinical Animal Research, requiring publishable-level data: n ≥ 10–12 per group, especially in models with large individual differences (such as high-fat, high-sugar induction models).

 

Summary Suggestions (Preliminary)

If it is an omics-type detection (such as metabolomics), the suggestions are as follows:

• Serum per tube: 100 – 200 μL

• Sample quantity per group: ≥ 8 mice

• 3 groups total: ≥ 24 mice, total serum volume required is approximately 2.4 – 4.8 mL

 

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