Are there any reagents and names needed for metabolomics?
The reagents commonly used in metabolomics are usually related to sample preparation, metabolite extraction, derivatization, and analysis. Here are some commonly used reagents and their names for different stages of metabolomics research for your reference:
1. Sample Preparation and Extraction:
- Methanol(Methanol): Widely used for sample pretreatment and extraction.
- Acetonitrile(Acetonitrile): Used for sample extraction and protein precipitation.
- Chloroform(Chloroform): Commonly used for the extraction of lipid metabolites.
2. Derivatization Reagents:
- Formamide(Formamide): Used for the derivatization of amino acids and other metabolites.
- Benzylamine chloride(Benzylamine chloride): Can be used for the derivatization of phenolic compounds.
3. Internal Standards:
- D4-Cyclodextrin(D4-Cyclodextrin): Used as an internal standard for standardization and calibration.
- 13C-Glucose(13C-Glucose): Can be used as an internal standard for carbon metabolism.
4. Analytical Reagents:
- Buffers(Buffers): Such as phosphate buffer and acetate buffer, used in liquid chromatography.
- Mass Spectrometry Calibration Solutions(Mass Spectrometry Calibration Solutions): Used for the calibration of mass spectrometers.
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