Protein Disulfide Bond Analysis

Disulfide bonds in proteins are a type of post-translational modification. Mass spectrometry can be used to identify and quantitatively analyze protein disulfide bonds. Biotech company Biotai Parker offers mass spectrometry-basedprotein disulfide bond analysisservices.

protein disulfide bonds

Disulfide bonds are a type of post-translational modification that occur during the biosynthesis of proteins in cells between the sulfur atoms of two cysteine residues. In biology, disulfide bonds are an important component of the secondary and tertiary structures of proteins and play a crucial role in protein function. Many proteins located on the cell surface or secreted extracellularly contain disulfide bonds, such as antibodies produced by the immune system, various growth factors, peptide hormones produced by the endocrine system like insulin, and the receptors for these growth factors or hormones. Analyzing disulfide bonds in proteins is significant for uncovering the higher structure and biological function of proteins. Additionally, incorrect disulfide bond formation or exchange can lead to antibody aggregation, making the understanding of disulfide bonds crucial in the characterization of proteins during biopharmaceutical production processes.

mass spectrometry analysis of protein disulfide bonds

Mass spectrometry, as an important technique in protein identification and structural-functional analysis, can be used for the analysis of protein disulfide bonds, including their identification, localization, and quantification. This can be applied to individual proteins or at the proteomics level. The basic steps include peptide extraction, peptide separation, and data analysis. During peptide extraction, it is important to minimize the chances of disulfide bond exchange in vitro to maintain the native protein structure as much as possible.

Related services:

Identification and quantitative analysis of protein disulfide bonds
Post-translational modification proteome analysis

protein disulfide bond analysis