Peptide Mass Spectrometry Analysis
Peptide mass spectrometry analysis is the process of analyzing and identifying peptide segments using mass spectrometry technology. These peptides can originate from a single polypeptide or protein, or from different polypeptides or proteins. BioPharm Solutions offers mass spectrometry-based peptide identification services.
Peptide segment
A peptide segment refers to shorter peptide chains obtained from the enzymatic digestion of polypeptides or proteins. A peptide is a shorter peptide chain formed by the dehydration condensation of two to fifty amino acids. Depending on the number of amino acids, peptides can be classified as oligopeptides or polypeptides. Proteins, as commonly referred to, are biological macromolecules formed by the combination of multiple polypeptides. In mass spectrometry analysis of polypeptides or proteins, it is generally necessary to first digest the polypeptides or proteins into shorter peptide segments before analysis. Therefore, whether analyzing polypeptide samples or protein samples, mass spectrometry is essentially analyzing peptide segments.
Peptide mass spectrometry analysis
Peptide mass spectrometry analysis involves the use of mass spectrometry technology to analyze and identify peptide segments. The peptides analyzed can originate from a single polypeptide or protein, or they can be mixtures of peptides from different sources. If the source polypeptide or protein of a single-origin peptide segment has a known gene sequence, cDNA sequence, or protein sequence, peptide mass fingerprinting (PMF) can be used for analysis and identification. PMF technology offers advantages of speed and high throughput compared to traditional methods. For peptide mixtures originating from different polypeptides or proteins, tandem mass spectrometry (MS/MS) is required for analysis and identification. MS/MS determines the mass of the peptide segment through primary mass spectrometry, and through secondary mass spectrometry, the peptide segment is dissociated into smaller peptide fragment ions. These fragment ions are analyzed by a detector to obtain the amino acid sequence information of the peptide segment, thereby achieving peptide identification.
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