Protein Post-Translational Modification Detection

Post-translational modification (PTM) detection includes the identification of protein post-translational modifications and the quantification of modified proteins. Biotech company Biotech Pack offers mass spectrometry-based services.Detection of protein post-translational modificationsservices.

Protein post-translational modifications

Specific sites on a protein's amino acid sequence can covalently bind with chemical groups or small molecular weight proteins, resulting in post-translational modifications (PTMs). PTMs are a step in protein biosynthesis, where the polypeptide chain undergoes modification before becoming a mature protein product. Types of PTMs include phosphorylation, acetylation, glycosylation, ubiquitination, and disulfide bonds, among others, which significantly increase proteome complexity. For any given protein, various PTMs provide a mechanism to promote rapid cellular changes by altering the protein's structure and function. PTMs play a crucial role in signal transduction, protein stability and turnover, protein recognition and interaction, and spatial localization.

Detection of protein post-translational modifications

Detection of protein post-translational modifications

Due to the significance of PTMs in fundamental biology and disease pathogenesis, there is a great need for the detection of protein post-translational modifications. Researching protein PTMs often requires enrichment steps, as these modified proteins are typically present in low relative abundance. Most PTM detection methods are developed in conjunction with enrichment strategies to provide optimal opportunities to identify, verify, and study the functions of PTMs. However, when detecting modified proteins with specific post-translational modification antibodies, enrichment steps may not be necessary. Mass spectrometry can detect PTMs by determining the molecular weight of peptide fragments, allowing for both qualitative and quantitative analysis of modified proteins.

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Proteomic analysis of post-translational modifications
Glycoprotein analysis