Plasma Protein Glycosylation Analysis

Plasma protein glycosylation analysis specifies qualitative or quantitative analysis of glycosylated proteins in plasma. Mass spectrometry can be used for plasma protein glycosylation analysis. Biotech company Biotyper provides mass spectrometry-based plasma protein glycosylation analysis services, including glycan analysis, glycosylation site identification, and glycoprotein quantification.

Plasma protein glycosylation

Protein glycosylation refers to the process by which carbohydrates are attached to proteins as a co-translational or post-translational modification. It is a common protein post-translational modification. Secreted extracellular proteins are often glycosylated, and glycosylated proteins are typically important integral membrane proteins. Proteins in plasma include those maintaining normal physiological states and proteins secreted, leaked, or shed from the body, among which some are glycosylated. Glycosylation modifications not only affect the spatial conformation, biological activity, transport, and localization of proteins but also play a crucial role in specific biological processes such as molecular recognition, cell communication, and signal transduction. Therefore, analyzing plasma protein glycosylation helps in studying the physiological functions of plasma proteins.

Plasma protein glycosylation analysis

Plasma protein glycosylation analysis

Plasma protein glycosylation analysis can be divided into qualitative and quantitative analysis. Qualitative analysis includes glycan analysis and glycosylation site analysis of glycosylated proteins. Mass spectrometry can be used for both qualitative and quantitative analysis of glycosylated proteins in plasma. To prevent high-abundance proteins in plasma from affecting the analysis of low-abundance proteins, plasma protein glycosylation analysis can first use liquid chromatography for separation and purification, followed by mass spectrometry analysis.

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