Shotgun Proteomics

Shotgun proteomics is a method for analyzing the proteome based on liquid chromatography and mass spectrometry techniques, overcoming the limitations and shortcomings of traditional gel separation techniques. Bio-tech company Biotree offers shotgun proteomics analysis services.

Shotgun Proteomics

Shotgun proteomics refers to the bottom-up proteomics technique that combines high-performance liquid chromatography with mass spectrometry to identify proteins in complex mixtures. The name is derived from shotgun DNA sequencing. The most common method in shotgun proteomics starts with proteins from the digested mixture, which are then separated by liquid chromatography into peptides, followed by peptide identification using tandem mass spectrometry. Shotgun proteomics allows for the identification of all proteins in a sample and has the ability to systematically analyze dynamic proteomes. It also avoids moderate separation efficiency and poor mass spectrometry sensitivity associated with whole protein analysis. Common quantitative proteomics analyses like label-free, SILAC, and iTRAQ are based on shotgun methods.

Shotgun Proteomics Workflow

In a shotgun proteomics experiment, proteins are first extracted from the sample and digested with proteases to produce a peptide mixture. The peptide mixture is then directly loaded onto a liquid chromatography column for separation. The separated peptides are ionized into ions and finally analyzed by tandem mass spectrometry (MS/MS). Using computer algorithms to analyze the obtained data, the measured peptides can be matched to corresponding proteins in a theoretical protein database, thus determining the protein composition of the sample.

Shotgun Proteomics

Related Services:

Quantitative Proteomics Analysis
Label-Free Quantitative Proteomics Analysis
SILAC/Dimethyl Labeling Based Quantitative Proteomics Analysis
TMT/iTRAQ/MultiNotch Quantitative Proteomics Analysis