Quantitative Mass Spectrometry Analysis
Quantitative mass spectrometry refers to the quantitative analysis of samples using mass spectrometry. Quantitative proteomics mass spectrometry involves the use of mass spectrometry technology for the quantitative analysis of the proteome. Biotech Pack offers quantitative proteomics analysis services based on mass spectrometry.
Quantitative mass spectrometry
Quantitative mass spectrometry refers to the quantitative analysis of samples using mass spectrometry. It can be used for the quantification of small molecule compounds, as well as for macromolecules like proteins. Here, we mainly introduce quantitative proteomics mass spectrometry.
Quantitative proteomics mass spectrometry
The abundance changes in the proteome within cells have a significant impact on life processes. For example, the occurrence and development of many diseases often accompany abnormal protein expression. Studying these abnormally expressed proteins can help us understand the mechanisms of disease occurrence and development.
Quantitative proteomics mass spectrometry utilizes mass spectrometry technology to accurately quantify and identify all proteins expressed by a genome or in a complex mixture system. Currently, quantitative proteomics mainly employs two strategies: label-based and label-free. Label-based strategies can be further divided into in vivo labeling (such as SILAC) and in vitro labeling (such as iTRAQ and TMT labeling). Label-free quantitative proteomics involves liquid chromatography-mass spectrometry (LC-MS) analysis of proteolytic peptides. This method compares the signal intensities of corresponding peptides in different samples to achieve relative quantification of the proteins corresponding to these peptides. In label-based quantitative proteomics, proteins in the sample are first labeled, and then relative quantification is achieved by comparing the peak areas of labeled versus unlabeled or differently labeled mass spectrometry peaks.
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