SILAC Proteomics

SILAC proteomics or SILAC quantitative proteomics refers to the quantitative study of proteomes using SILAC technology. Biotyper Biotech providesQuantitative proteomic analysis based on SILAC labelingservices.

SILAC

In mass spectrometry (MS)-based quantitative proteomics, SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) is a method where stable isotope-labeled amino acids are added during cell culture to label samples, which are then analyzed by mass spectrometry. It is a simple, reliable, and powerful method for proteomic analysis. SILAC labels cellular proteins through normal metabolic processes, incorporating non-radioactive, stable isotope-containing amino acids into newly synthesized proteins. SILAC offers accurate relative quantification without any chemical derivatization or manipulation, enabling proteomic analysis. It is an in vivo labeling technique that keeps samples close to their natural state, with labeling efficiency reaching 100% and stable labeling effects, suitable for whole-cell protein analysis as well as identification and quantification of membrane proteins, with each sample requiring only a few micrograms of protein.

SILAC proteomic analysis

The experimental workflow of SILAC quantitative proteomic analysis involves adding light, medium, or heavy stable isotope-labeled essential amino acids lysine (Lys) and arginine (Arg) to the cell culture medium. During cell growth, newly synthesized proteins incorporate stable isotope tags. After culturing for a period, proteins from mixed cell cultures are equally combined, digested, and analyzed by mass spectrometry. Due to mass differences, peptide pairs with identical chemical properties but different stable isotopic compositions can be distinguished in the mass spectrometer. Relative quantification is achieved by comparing the area sizes of mass spectrometry peaks from the first stage spectrum of unlabeled and isotope-labeled samples. Additionally, the second stage spectrum allows for peptide sequencing and protein identification.

SILAC proteome

related services

Quantitative proteomic analysis based on SILAC/Dimethyl labeling
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