Quantitative Proteomics

Quantitative proteomics is a discipline that studies protein content and its changes. BGI Pathology provides mass spectrometry-basedquantitative proteome analysisservices.

Quantitative proteomics

Quantitative proteomics involves the precise quantification and identification of all proteins expressed by a genome or within a complex mixture. It is an essential branch of proteomics research. The expression levels of proteins are closely related to biological activities, making quantitative analysis of proteins crucial for elucidating their biological functions and various cellular processes.

Quantitative proteomics research techniques

Currently, common methods for quantitative proteomics can be divided into fluorescence-based quantitative techniques, protein microarray technology, and mass spectrometry-based quantitative techniques.

Fluorescence-based quantitative technology combines two-dimensional gel electrophoresis with fluorescence staining techniques. It uses fluorescent dyes to label proteins, separates and identifies them on 2D gels, and compares protein amount variations based on differences in fluorescence intensity. Protein microarray technology captures ligands from test samples using protein chips and performs quantitative proteome analysis using laser confocal microscopy or mass spectrometry.

Mass spectrometry-based protein quantitative techniques measure relative quantities by comparing the peak intensities of proteins or peptides with similar ionization capabilities. Compared to other techniques, this method can determine peptide sequences and accurately quantify proteins. Currently, mass spectrometry-based quantitative proteomics is mainly divided into labeled and label-free methods. Labeled methods can further be divided into in vivo labeling (such as SILAC) and in vitro labeling (such as iTRAQ, TMT labeling).

Quantitative proteomics

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quantitative proteome analysis