Top Down Method-Based PTMs Characterization
Top-down mass spectrometry (MS) can comprehensively analyze protein post-translational modifications (PTMs) because it analyzes intact proteins or peptides without the need for hydrolysis. Therefore, in the analysis of protein biologics such as monoclonal antibodies and recombinant proteins, this strategy can retain most of the unstable modification information that is lost when using CID fragmentation analysis under the shotgun approach.
Zhang, H. et al. Circ Cardiovasc Genet. 2011.
Characterization of PTMs based on top-down approach
Top-down mass spectrometry using ECD and ETD has unique advantages in determining the mass of intact proteins or peptides, quantifying various modified protein forms, mapping modification patterns with high coverage, identifying unpredicted PTMs, and determining the sequence of multiple modifications. Although top-down proteomics is still in its infancy and faces technical challenges in sample preparation and throughput, these unique advantages make it an extremely important analytical approach for characterizing post-translational modifications.
Biotech company Biotree has experienced top-down proteomics scientists and technicians who provide you with high-quality PTMs characterization services.
Related services
Post-translational modification proteome analysis
How to order?
