How to choose between Label free, iTRAQ, TMT, SILAC, and SWATH protein quantification technologies?
Label-free, iTRAQ, TMT, SILAC, and SWATH are the mainstream mass spectrometry-based protein quantification techniques currently available. Each of these five techniques has different principles and advantages, and one can choose based on specific needs in practical applications. Label-free quantification is relatively inexpensive but cannot avoid systematic errors, resulting in slightly lower quantification accuracy. Both iTRAQ and TMT are in vitro isotope labeling quantification techniques with similar principles, developed by different companies, and differ in the number of tags. iTRAQ and TMT can perform differential quantification of total proteins in any sample and provide accurate quantification. SILAC is an in vivo isotope labeling technique that can only be used for live, proliferative cells or bacterial samples, and it takes a bit longer. SWATH technology offers high throughput, strong reproducibility, and high data accuracy; its only downside might be the cost.
How to order?
