What types of buffers and solutions are compatible with peptide purification and LC-MS?
Most organic solvents or volatile buffers are compatible with LC-MS. Water and acetonitrile containing 0.1% formic acid are the most commonly used solvent systems for separating peptide samples in mass spectrometry analysis. Trifluoroacetic acid (TFA) is an alternative ion-pairing agent used for offline peptide C18 purification or LC-UV analysis.
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