What are the differences between MS and NMR based metabolomics analysis?
The key difference lies in sensitivity. Mass spectrometry (MS) is several orders of magnitude more sensitive than nuclear magnetic resonance (NMR). In NMR-based metabolomics analysis, such as human plasma, measurements typically cover 200-300 predefined compounds present at high concentrations in the sample. In MS-based metabolic profiling, all chemical signals produced by thousands of compounds present in the sample are considered in the analysis. The MS method includes a broader compound library in the analysis, including unknown compounds and those at low concentrations. These two methods are mostly complementary because some compounds are better detected using NMR.
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