Unknown Protein Identification
The identification of unknown proteins refers to the process of recognizing, studying, and annotating proteins whose functions and structures have not yet been fully understood, mainly includesprotein molecular weight determination,amino acid sequence determination, structure prediction, function analysis, and interaction network analysis, etc..Mass spectrometry is an important method for identifying unknown proteins, and through the identification of unknown proteins, we candiscover new biomarkers, drug targets, and therapeutic strategies, providing important information for the prevention, diagnosis, and treatment of diseases.
BioTechPack BTPbased onthe latest Obitrap Exploris 240 mass spectrometer by Thermo combined with Nano-LC nanoliter chromatographyfor unknown protein identification services, can provide efficient and precise mass spectrometry identification services for unknownproteins in protein extracts, SDS-PAGE protein bands, 2D protein gel spots, pull-down, and Co-IP samples, including the molecular mass, amino acid composition and sequence arrangement, and post-translational modification information of unknown proteins..
BioTechPackBTPUnknown Protein IdentificationProcess
1.ProteinSample Preparation
2. Protein Separation and Purification
3.Protein Mass Spectrometry Analysis
4. Protein Sequence Analysis
5.Structure Prediction
6.Function Analysis
7.Interaction Network Analysis
OurAdvantages
1. Advanced Mass Spectrometry Equipment:
We have the latest high-resolution mass spectrometers,includingthe Q-Exactive series and Orbitrap series, which can provide you with high-quality, high-throughput mass spectrometry data, ensuringhigh accuracy of amino acid sequence information, enabling accurate identification of unknown proteins.
2. High Sensitivity, High Throughput:
Can simultaneously analyze multiple proteins or peptides, and candetect very low abundance proteins, meeting the needs for identifying rare or low abundance proteins.Demand.
3. Rich Protein Separation Techniques:
Our team is experienced in protein separation and can select appropriate separation methods such as 1D or 2D-PAGE, HPLC, etc., based on sample characteristics to reduce sample complexity and improve protein identification coverage.
4. Efficient Enzymatic Digestion and Peptide Separation:
We use optimized enzymatic digestion conditions and multidimensional liquid chromatography techniques to effectively improve the identification accuracy and coverage of peptides.
5. Broad Applicability:
Can analyze proteins from different sources and types, includingbut not limited tobacteria, viruses, eukaryotes, and complex biological samplesetc..
Unknown Protein IdentificationMain Applications
1. Discovery of New Proteins:
Through unknown protein identification technology, new protein sequences can be identified.
2. Protein Function Research:
Revealing the sequence information of unknown proteins helps to gain a deeper understanding of their functions and roles in cells and organisms.
3. Protein Modification Research:
Identifying the types and positions of protein modifications, such as phosphorylation and methylation, helps to understand the regulatory mechanisms of protein functions.
4. Drug Development:
Can be used to identify target proteins for drug action, helping to accelerate the development and optimization of new drugs.
5. Diagnosis and Treatment:
Can help identify new biomarkers, which is important for early diagnosis and treatment of diseases.
Chinese/English Project Report
In the technical report, BioTechPack will provide you with a detailed bilingual (Chinese/English) technical report, including:
1. Experimental Steps (Chinese/English)
2. Relevant Mass Spectrometry Parameters (Chinese/English)
3. Detailed Information on Unknown Protein Identification
4. Mass Spectrometry Images
5. Raw Data
One-Stop Service for Unknown Protein Identification
You only need to place an order and send the samples
BioTechPack's one-stop service completes: sample processing - on-machine analysis - data analysis - project report
Related Services
Protein Mass Spectrometry Identification
Membrane Protein Identification Service
Protein Structure Identification
Protein Molecular Weight Determination
Protein Gel Spot, Gel Band, IP Sample Protein Identification
Full Protein Sequence Determination
Quantitative Proteomics Analysis
Post-Translational Modification Proteomics Analysis
Protein-Protein Interaction Mass Spectrometry Analysis
How to order?
