Protein Characterization Analysis
Proteins are the carriers of life substances and executors of biological functions, playing a crucial role in maintaining normal life activities of the organism.Protein characterization analysis(Protein characterization analysis) includes the characterization of protein purity, molecular weight, structure, post-translational modifications, and interactions.As one of the core fields of biological science research, protein characterization analysis reveals how proteins function in biological systems and how they interact to maintain normal physiological functions.
Common methods for protein characterization analysis include Nuclear Magnetic Resonance (NMR),,Mass Spectrometry (MS), andWesternBlotting (Western blot), etc. Nuclear Magnetic Resonance (NMR) is commonly usedforstudying the three-dimensional structure and dynamic properties of proteins. Through Mass Spectrometry (MS) technology, one can analyze the protein'smolecularmass,post-translationalmodifications, and interactions.WesternBlotting (Western blot) uses specific antibodies to detect the distance and interactions between proteins.
BiotechPackard BTPProtein Characterization AnalysisServices
1. Protein Purity Analysis
Various purity analysis methods: SDS-PAGE, capillary electrophoresis, zinc reverse staining, non-glutaraldehyde modified silver staining, and MALDI-TOF mass spectrometry,to detect contaminants, protein variants, isomers, S-S bond mismatches, truncated protein products, degraded proteins, protein modifications, protein aggregates, and protein precursors.
2. Protein Molecular Weight Analysis
Determination of the molecular weight of protein complexes and protein-small molecule complexes using gel permeation chromatography, SDS-PAGE, and mass spectrometry.
3. Protein Structure Identification
Based on Thermo Fisher's Orbitrap Fusion Lumos mass spectrometer platform combined with nanoLC-MS/MS nano liquid chromatography for top-down protein primary structure characterization (includingamino acid composition analysis ,peptide sequence analysis , anddisulfide bond localization , etc.), and based oncircular dichroism spectroscopy for protein spatial conformation determination.
4. Protein Post-Translational Modification Analysis
Multiple mass spectrometry platforms (Thermo Fisher's Q ExactiveHF, Orbitrap Fusion, Orbitrap Fusion Lumos combined with Nano-LC) for identifying protein phosphorylation/glycosylation/ubiquitination/acetylation/methylation/disulfide bonds/nitrosylation and other post-translational modifications.
5. ProteinInteractionAnalysis and Interaction Protein Identification
Using Western blotting, immunoprecipitation, (GST) Pull-down, etc., to study protein-protein interactions, andbased on LC-MS/MS technology for mass spectrometry identification analysis services of proteins/protein mixtures in IP, Co-IP samples, and GST fusion protein Pull-down purified samples.
InEnglish Project Reports
In the technical report, BiotechPackard provides you with a detailed bilingual technical report in Chinese and English, including:
1.Experimental Procedures (in Chinese and English)
2.Relevant Mass Spectrometry Parameters (in Chinese and English)
3.Protein Characterization AnalysisDetailed Information
4.Mass Spectrometry Images
5.Raw Data
Protein Characterization AnalysisOne-Stop Service
You only need to place an order and send the samples
BiotechPackard completes the one-stop service: sample processing - machine analysis - data analysis - project report
Related Services
Protein Mass Spectrometry Identification
Protein Structure Identification
Protein Circular Dichroism Analysis
Protein Molecular Weight Determination
Amino Acid Composition Analysis
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