Serum Protein Group Analysis
Serum is bloodThe transparent yellow fluid obtained after a blood sample is coagulated and centrifuged, removing blood cells and coagulation factors, mainly composed ofwater, proteins (albumin, fibrinogen, and globulins), lipids, carbohydrates, and other substances. The proteins in serum contain rich biological information, which is of significant importance in the occurrence, development, prediction, and diagnosis of diseases. Serum proteomics is a method to study the types, structures, and functions of proteins in serum, used to gain insights into biological processes, disease mechanisms, and drug targets. Serum proteomics is a promising research method, and as technology advances, it will play an increasingly important role in future biomedical research.
The main technologies for serum proteome analysis include protein separation and purification techniques, such as gel electrophoresis, liquid chromatography, etc.;protein identification techniques, such as mass spectrometry,Edmandegradation method, etc.;protein quantification techniques, such as labeling methods,non-labeling methods, etc.;and bioinformatics analysis, such as protein clustering analysis, functional annotation, and pathway analysis. These methodologies complement each other, collectively driving the development of serum proteome analysis.
BTP BiotechSerum Proteome AnalysisGeneral Process
1.SamplePreparation and Processing
Centrifuge to separate serum from the sample, removing red blood cells, white blood cells, and platelets, then treat the sample with protease inhibitors to prevent protein degradation.
2. ProteinExtraction and Purification
Extract and purify proteins from serum using ammonium sulfate precipitation, ion-exchange chromatography, affinity chromatography, etc..
3. Protein Component Separation
Separate protein components using one-dimensional or two-dimensional gel electrophoresis, liquid chromatography, etc..
4. ProteomeIdentification andQuantification
Mass spectrometry can provide information on molecular weight and peptide sequences to achieve proteome identification and quantification.
5. Data Processing and Analysis
Usesoftware such as Mascot, SEQUEST, MaxQuantto process and analyze the obtained mass spectrometry data.
6. Bioinformatics Analysis
Integrate analysis results with known bioinformatics data,to reveal the role of the proteome in biological processes and diseases.
Serum Proteome AnalysisTechnology Applications
1. DrugTarget Screening and DrugDevelopment
2. Disease DiagnosisandBiomarker Research
3.SystemsBiology Research
Bilingual Project Report
In the technical report, BTP will provide you with a detailed bilingual (Chinese and English) technical report, which includes:
1. Experimental Steps (Chinese and English)
2. Related Mass Spectrometry Parameters (Chinese and English)
3.Serum ProteomeAnalysis Details
4. Mass Spectrometry Images
5. Raw Data
Serum ProteomeAnalysis One-Stop Service
All you need to do is place an order and send the sampleBTP's one-stop service completes: sample processing - on-machine analysis - data analysis - project report
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