HPLC Peptide Molecular Weight Determination

The molecular weight is a basic physicochemical parameter of a compound, referring to the sum of the relative atomic masses of the atoms composing the compound. It is also one of the important characteristic parameters of proteins/polypeptides, playing a crucial role in the identification of proteins/polypeptides. The correctness of the molecular weight is also an important indicator for determining the correctness of its structure. Common methods for identifying the molecular weight of proteins/polypeptides include SDS-PAGE, viscosity method, gel permeation chromatography, gel filtration chromatography, and mass spectrometry.

Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC) are both High Performance Liquid Chromatography (HPLC) techniques. Their principle for identifying molecular weight is similar, both based on separating the components in a mixture according to the size of the protein/polypeptide molecules. The chromatography column is filled with a porous gel molecular sieve. When the mixed sample flows through the chromatography column, components with larger molecular weights cannot penetrate the gel pores and can only pass through the gaps between the gels, thus eluting first. Components with smaller molecular weights can enter the gel pores and thus take longer to elute. By measuring the elution time and volume of each component and comparing them with standard samples, the relative molecular weight of each component can be calculated, and its molecular weight distribution can be estimated.

Based on an advanced chromatography platform combined with a professional technical team, Biotage Parker Biotechnology provides efficient and rapidHPLC protein purity identificationservicestechnology package, which can be used for the separation and determination of various protein/polypeptide samples to meet the needs of protein/polypeptide sample purity analysis. Welcome to consult for free.

Related services:
Protein molecular weight determination
Determination of the molecular weight of protein complexes, protein and small molecule complexes
High-resolution mass spectrometry molecular weight identification
MALDI TOF mass analysis
Accurate mass measurement