Protein Separation, Purification and Identification Comprehensive Experiment

In proteomics research, to perform qualitative or quantitative analysis of proteins in a mixed system, it is necessary to first separate and purify the protein components, and then carry out the subsequent identification analysis. This series of experiments is interconnected, and each step is crucial, directly affecting the accuracy of the experimental results.

An ideal protein separation technology should first have ultra-high resolution, capable of effectively separating thousands of different types of proteins, including their modifications. Currently, commonly used protein separation technologies include one-dimensional/two-dimensional gel electrophoresis, two-dimensional electrophoresis, and gel chromatography.

The separated proteins need to undergo purity testing to evaluate whether they contain other contaminant proteins or impurities. Common methods for purity testing include polyacrylamide electrophoresis, immunochemical methods, sedimentation rate measurement, chromatography, and mass spectrometry. Proteins containing impurities need to be purified. For example, impurities such as nucleic acids, carbohydrates, or lipids can be removed using nucleic acid precipitation or organic solvent precipitation.

Protein identification is mainly based on its basic physicochemical parameters, including relative molecular weight, isoelectric point, post-translational modifications, amino acid sequence, and higher-order structures. One can choose to identify a single property or conduct a comprehensive analysis based on experimental needs.

Biotech Peaker providesone-stop technical services for protein separation, purification, and identificationbased on advanced mass spectrometers and a professional technical team, including protein sample separation, purification, purity identification, and qualitative and quantitative identification. Customized analysis services are also available to meet different experimental needs. Free consultation is welcome.

Related services:
Protein separation based on SDS-PAGE
SDS-PAGE protein purity analysis
Protein purity analysis (size exclusion/reverse phase chromatography)
Protein mass spectrometry identification
Protein identification for gel spots, gel strips, and IP samples
Protein structure identification
Determination of protein primary structure